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中国农学通报

中国农学通报 ›› 2008, Vol. 24 ›› Issue (10): 445-449.

所属专题： 园艺

• 植物保护 农药 • 上一篇    下一篇

嗜铁素产生菌ZJH-10诱导黄瓜抗灰霉病的研究

梁建根   

  • 收稿日期:2008-08-15 修回日期:2008-08-20 出版日期:2008-10-05 发布日期:2008-10-05

Induced Resistance to Gray Mould in Cucumber by Siderophore-Producing Bacterium ZJH-10

LIANG Jian-Gen   

  • Received:2008-08-15 Revised:2008-08-20 Online:2008-10-05 Published:2008-10-05

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737

摘要： 为研究嗜铁素产生菌ZJH-10诱导黄瓜对灰霉病的抗性的作用机制，采用生理生化的方法，测定了黄瓜根部与叶部组织中的超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）、多酚氧化酶（PPO）和苯丙氨酸解氨酶（PAL）系列防御酶活性变化。结果表明，黄瓜经ZJH-10处理并同时接种灰霉病菌后，黄瓜根部与叶部组织中SOD、POD、CAT、PPO和PAL活性都明显高于其它处理。其根部活性分别在处理后 5d、3d、5d、3d和3d出现高峰，叶部酶活性变化基本与根部的相吻合。表明这些酶活性的提高与ZJH-10诱导的黄瓜抗灰霉病抗性可能有一定的相关性。

关键词: 八倍体小偃麦, 八倍体小偃麦, 普通小麦, 远缘杂交, 小麦新品种

Abstract: In order to explore the mechanism of the resistance to gray mould induced by siderophore-producing bacterium ZJH-10, the physiological and biochemical methods were employed to study the changes in activities of superoxide dismutase(SOD), peroxidase(POD), catase(CAT), polyphenol oxidase(PPO) and phenylalanine ammonia-lyase(PAL) in cucumber roots and leaves pretreated with siderophore-producing bacterium ZJH-10 and infected by Botrytis Cinerea. The results showed that the activities of SOD, POD, CAT, PPO and PAL in the roots treated by ZJH10 increased higher than those of other treatments to some extent and reached their maximum on 5th, 3rd, 5th,3rd and 3rd in roots, respectively, the activities of these protective enzymes in leaves were accorded with those of enzymes in roots. The experimental results suggested that the increased activities of SOD、POD、CAT、PPO、and PAL induced by ZJH-10 against Botrytis Cinerea are most likely to be associated with the development of ISR in cucumber.