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中国农学通报

中国农学通报 ›› 2021, Vol. 37 ›› Issue (9): 16-21.doi: 10.11924/j.issn.1000-6850.casb2020-0205

所属专题： 生物技术

• 农学·农业基础科学 • 上一篇    下一篇

金花葵染色体倍性分析及属间远缘杂交研究

尹国(), 路正营, 孙璐, 张彦波, 李世云()   

  1. 邯郸市农业科学院,河北邯郸 056001
  • 收稿日期:2020-06-24 修回日期:2020-08-27 出版日期:2021-03-25 发布日期:2021-04-09
  • 通讯作者: 李世云
  • 作者简介:尹国,男,1981年出生,河北邱县人,副研究员,硕士,研究方向：主要从事棉花分子育种研究。通信地址：056001 河北省邯郸市邯山区农研路1号 邯郸市农业科学院,Tel：0310-8160516,Email： yinguo7514053@163.com。
  • 基金资助:
    国家棉花产业技术体系“冀南综合试验站”(CARS-15-30);河北省重点研发计划“早熟优质多抗棉花种质创新及新品种培育”(20326314D)

Chromosome Ploidy Analysis and Interspecific Hybridization of Abelmoschus manihot

Yin Guo(), Lu Zhengying, Sun Lu, Zhang Yanbo, Li Shiyun()   

  1. Handan Academy of Agriculture Science, Handan Hebei 056001
  • Received:2020-06-24 Revised:2020-08-27 Online:2021-03-25 Published:2021-04-09
  • Contact: Li Shiyun

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摘要：

为了研究‘金花葵3号’的染色体倍性,以‘金花葵3号’新生根尖为材料,采用常规压片法,对‘金花葵3号’进行染色体倍性分析,显示金花葵为二倍体,共含有66条染色体,核型公式为K(2n)=2x=66。以‘金花葵3号’和含有52条染色体的异源四倍体陆地棉‘邯818’为父母本进行杂交,并进行反交,结果显示：以‘金花葵3号’为母本的杂交组合可以得到杂交种F1,以其gDNA为模板PCR扩增检测结果为含有双亲特异性条带的杂合条带,杂交种可正常发育,但是花粉败育,无法结实;以‘金花葵3号’为父本的杂交组合幼铃全部脱落,未得到杂交种。以杂交种F1新生根尖为材料,压片结果显示其含有59条染色体,进一步证实杂交种F1为双亲杂交所得。本研究首次揭示金花葵这一物种的染色体倍性及其属间杂交的遗传规律,为进一步研究金花葵的细胞遗传学提供了理论依据。

关键词: 金花葵, 倍性分析, 棉花, 远缘杂交, 属间杂交

Abstract:

To study the chromosome ploidy of ‘Abelmoschus manihot No.3’, using the new root tip of ‘Abelmoschus manihot No.3’ as material, the chromosome ploidy analysis was carried out by conventional squash method. The results showed that ‘Abelmoschus manihot No.3’ was diploid with 66 chromosomes, and the karyotype formula was K (2n)=2x=66. The hybrid F1 could be obtained by crossing ‘Abelmoschus manihot No.3’ and allotetraploid upland cotton ‘Han 818’ with 52 chromosomes. The results showed that the hybrid F1 could be obtained from the cross combination with ‘Abelmoschus manihot No.3’ as female parent. The PCR amplification results showed that the hybrid contained parental specific bands. The hybrid could develop normally, but the pollen was abortive and could not bear. All the young bolls of the hybrid combinations with ‘Abelmoschus manihot No.3’ as male parent fell off, and no hybrid was obtained. The results of squash showed that there were 59 chromosomes in the new root tip of F1 hybrid, which further confirmed that F1 hybrid was obtained by crossing parents. This study revealed for the first time the genetic regularity of chromosome ploidy and intergeneric hybridization in this species, which could provide a theoretical basis for further study on cytogenetics of Abelmoschus manihot .

Key words: Abelmoschus manihot, ploidy analysis, cotton, distant hybridization, intergeneric hybridization

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