中国农学通报 ›› 2009, Vol. 25 ›› Issue (1): 237-240.
• 农学 农业基础科学 • 上一篇 下一篇
吕伟成,张绍升
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摘要： 摘要：通过设计尖孢镰刀菌ITS区特异引物PR1/PR2和SCAR特异引物ST1/ST2，优化检测体系中反应参数，建立了双重PCR检测香蕉枯萎病菌1号生理小种和4号生理小种的方法。结果表明：引物PR1/PR2和ST1/ST2能明确鉴别香蕉枯萎病菌1号生理小种和4号生理小种，检测体系的最佳退火温度为56 ℃，检测灵敏度的最低起始DNA为20 ng。
关键词: 雷蘑（Clitocybe gigantean）AS 5.105, 雷蘑（Clitocybe gigantean）AS 5.105, 胞外多糖, Plackett-Burman设计, 响应曲面法(RSM), Box-Behnken设计
Abstract: Abstract： Fusarium oxysporum f.sp.cubense(Foc) is the most destructive pest of bananas in the world. Two pairs of primers PR1/PR2 and ST1/ST2 were developed to identify the Foc. The primers PR1/PR2 and ST1/ST2 could produce bands for race1 and race4 of Foc. At the same time, the parameters of PCR were optimized and sensitivity were tested. The optimized conditions were an annealing at 56 ℃. The minimum amount of DNA of Foc could be detected was as low as 20 ng. It demonstrates that duplex PCR approach will provided an accurate and effective method for quick detection of Foc.
中图分类号:
S436.67
吕伟成,张绍升. 一步双重PCR检测香蕉枯萎病菌[J]. 中国农学通报, 2009, 25(1): 237-240.
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