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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (1): 241-247.

所属专题： 生物技术； 园艺

• 农学 农业基础科学 • 上一篇    下一篇

巴西橡胶树小橡胶粒子蛋白（SRPP）基因启动子的克隆及其功能分析

李先昆,段翠芳,聂智毅,吴华玲,黎瑜,曾日中   

  • 收稿日期:2008-09-22 修回日期:2008-11-10 出版日期:2009-01-05 发布日期:2009-01-05

Cloning and functional analysis of the promoter of small rubber particle protein (SRPP) gene in Hevea brasiliensis

  • Received:2008-09-22 Revised:2008-11-10 Online:2009-01-05 Published:2009-01-05

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摘要： 摘要：小橡胶粒子蛋白（SRPP）是巴西橡胶树中参与橡胶生物合成的重要蛋白因子之一。SRPP的氨基酸序列与橡胶延长因子（REF）和菜豆胁迫相关蛋白（PVSRP）的同源性分别为72%和68%。为了进一步研究SRPP基因表达及其调控的机制，作者采用接头连接介导的PCR散步法（ligation-mediated PCR），克隆了SRPP基因的启动子。序列分析表明，SRPP基因启动子中与光诱导相关的顺式元件占39%，可能属于光诱导型启动子，因此，SRPP基因的表达可能受光信号的调控。此外，SRPP基因启动子还具有热激响应元件（HSE）、干旱胁迫响应元件（MBS）、防卫和胁迫响应元件（TC-rich repeats）、脱落酸响应元件（ABRE）、赤霉素响应元件（GARE）和激发子响应元件（EIRE，W box）等，这表明橡胶树SRPP基因不仅参与调控橡胶生物合成，而且可能是橡胶树中响应逆境信号的抗性基因，在橡胶树抵御逆境胁迫的生理过程中发挥重要作用。

关键词: 烤烟, 烤烟, 漂浮育苗, 基质, 漂浮育苗专用肥, 播期, 剪叶

Abstract: Abstact: The small rubber particle protein (SRPP) is an essential factor related to rubber biosynthesis in Hevea brasiliensis (rubber tree). The amino acid sequence of SRPP has high similarity to those of rubber elongation factor (REF) and phaseolus vulgaris stress-related protein (PvSRP), which is 72% and 68% respectively. For further investigation of the expression of SRPP gene, the promoter of SRPP gene was cloned by the method of ligation-mediated PCR. Sequence analysis demonstrated that light-responsive elements accounted for 39% in the promoter of SRPP gene, which indicates that SRPP promoter might be light inducible and that the expression of SRPP gene could be regulated by light. In addition, the promoter of SRPP gene contains a variety of stress-responsive elements, such as HSE,MBS, TC-rich repeats, ABRE, EIRE and W box etc. These data suggest that SRPP is not only involved in rubber biosynthesis, but also plays an important role in response to various stresses in rubber trees.