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中国农学通报

中国农学通报 ›› 2017, Vol. 33 ›› Issue (22): 36-40.doi: 10.11924/j.issn.1000-6850.casb17040160

所属专题： 食用菌

• 生物技术科学 • 上一篇    下一篇

舞茸菌丝体与胞外多糖液体培养基的优化

刘晓柱,李银凤,赵晓伟,赵燕   

  1. 贵州理工学院,贵州理工学院,贺州学院,湖南农业大学
  • 收稿日期:2017-04-26 修回日期:2017-07-19 接受日期:2017-06-12 出版日期:2017-08-21 发布日期:2017-08-21
  • 通讯作者: 赵燕
  • 基金资助:
    湖南省自然科学基金项目“乳球菌α-半乳糖苷酶基因克隆及其工程改造”(2014FJ3082)。

Effects of Medium Nutrition on Growth of Grifola frondosa Mycelium and Production of Extracellular Polysaccharides

  • Received:2017-04-26 Revised:2017-07-19 Accepted:2017-06-12 Online:2017-08-21 Published:2017-08-21

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摘要： 以舞茸(Grifola frondosa) LT菌株为材料，研究培养基营养成分对菌丝体和胞外多糖的影响。通过单因素实验确定葡萄糖、蛋白胨、磷酸二氢钾及维生素B1为最适宜的碳源、氮源、无机盐和生长因子。多因素正交实验进一步优化上述组分的浓度，确定舞茸菌丝体最适宜的发酵培养基配方为：10%豆芽汁200 mL，葡萄糖20 g/L、蛋白胨5 g/L、磷酸二氢钾4 g/L、维生素B1 30 mg/L，pH 7.0；胞外多糖最适宜的发酵培养基配方为:10%豆芽汁200 mL，葡萄糖20 g/L、蛋白胨3 g/L、磷酸二氢钾4 g/L、维生素B1 30 mg/L，pH 7.0。本研究为进一步开展舞茸菌丝体和胞外多糖液体发酵与利用提供了理论参考。

关键词: 杉木, 杉木, γ射线, 诱变育种, 种子萌发, 幼苗生长

Abstract: In the present study, a strain of Grifola frondosa LT was used to evaluate the effects of medium nutrition on the growth of mycelium and production of extracellular polysaccharides. Glucose, peptone, monopotassium phosphate and vitamin B1 were established as the optimal carbon source, nitrogen source, inorganic salt and growth factor via single factor test. The concentration of medium nutrition was further optimized through multi-factor orthogonal test, and the optimum fermentation condition for the growth of mycelium were 10% of bean sprouts 200 mL, glucose 20 g/L, peptone 5 g/L monopotassium phosphate 5 g/L and vitamin B1 30 mg/L, and the optimum fermentation condition for production of extracellular polysaccharides were 10% of bean sprouts 200 mL, glucose 20 g/L, peptone 3 g/L monopotassium phosphate 4 g/L and vitamin B1 30 mg/L. This research was of certain values to further fermentation researches and applications of Grifola frondosa mycelium and polysaccharides.