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中国农学通报

中国农学通报 ›› 2021, Vol. 37 ›› Issue (36): 24-29.doi: 10.11924/j.issn.1000-6850.casb2021-0221

• 农学·农业基础科学 • 上一篇    下一篇

外源锌对氮胁迫下薏苡幼苗生长及抗氧化系统的影响

熊露露(), 卢囿佐, 刘月炎, 彭海兰, 黄兴敏, 王健健()   

  1. 贵州大学生命科学学院/农业生物工程研究院,山地植物资源保护与保护种质创新教育部重点实验室,山地生态与农业生物工程协同创新中心,贵阳 550025
  • 收稿日期:2021-03-05 修回日期:2021-06-13 出版日期:2021-12-25 发布日期:2022-02-15
  • 通讯作者: 王健健
  • 作者简介:熊露露,女,1996年出生,贵州印江人,硕士研究生,研究方向：植物生态学。通信地址：550025 贵州省贵阳市花溪区贵州大学,Tel：0851-83859885,E-mail： 1034272561@qq.com。
  • 基金资助:
    国家自然科学基金资助项目“喀斯特地区典型药用植物对气候变化的响应及其调控机制研究”(31760155);贵州省高层次创新型人才项目(黔科合平台人才[2020]6005)

Exogenous Zinc: Effects on the Growth and Antioxidant System of Coix lachryma-jobi L. Under Nitrogen Stress

XIONG Lulu(), LU Youzuo, LIU Yueyan, PENG Hailan, HUANG Xinmin, WANG Jianjian()   

  1. Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Collaborative Innovation Center for Mountain Ecology & Agro-Bioengineering (CICMEAB), College of Life Sciences/Institute of Agro-Bioengineering, Guizhou University, Guiyang 550025
  • Received:2021-03-05 Revised:2021-06-13 Online:2021-12-25 Published:2022-02-15
  • Contact: WANG Jianjian

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摘要：

为缓解薏苡氮胁迫伤害,为缺氮地区薏苡栽培提供参考依据。以贵州省黔西南薏苡种子为试验材料,采用盆栽试验方法,测定分析外源锌与氮胁迫对薏苡幼苗形态生长、叶绿素含量、生物量积累与分配、过氧化氢酶(CAT)、过氧化物酶(POD)活性及丙二醛(MDA)积累的影响。结果表明,（1）缺氮时薏苡幼苗株高、根长、主根数、总叶片数及各部分生物量显著下降（P<0.05,下同）,致使总生物量较对照显著降低80.65%,CAT、POD活性受到显著抑制,MDA含量显著增加26.29%。（2）施锌显著增加了薏苡株高、叶面积及茎生物量,叶绿素含量整体呈上升趋势,CAT、POD活性显著提高;同时,根系对外源锌表现较为敏感,其根长显著降低28.09%,而主根数显著增加16.86%;（3）缺氮施锌显著提高缺氮薏苡幼苗株高和根长,增幅分别为20.30%、17.72%,抗氧化酶CAT活性显著提高171.01%,而对POD活性及MDA积累影响不大。研究认为,氮胁迫下施锌能促进薏苡幼苗生长和抗氧化系统的平衡,提高薏苡幼苗的适应能力,在一定程度上缓解了氮胁迫所导致的不良影响。

关键词: 薏苡, 氮胁迫, 外源锌, 幼苗生长, 抗氧化系统

Abstract:

The study aims to alleviate the injury of nitrogen stress on Coix lachryma-jobi L. and provide a reference basis for its cultivation in nitrogen-deficiency areas. Taking the seeds of Coix lachryma-jobi L. in southwest Guizhou as test materials, pot experiment method was used to study the effects of exogenous zinc and nitrogen stress on plant growth, chlorophyll content, biomass accumulation and distribution, peroxidase (POD) and catalase (CAT) activities, and malondialdehyde (MDA) content of Coix lachryma-jobi L. seedlings. The results showed that: (1) the plant height, root length, main root number, total leaf number and biomass of each part of Coix lachryma-jobi L. seedlings decreased significantly under nitrogen deficiency (P<0.05, the same below), which decreased the total biomass by 80.65% compared with that of the control; the activities of CAT and POD were significantly inhibited, and the content of MDA significantly increased by 26.29%; (2) zinc application significantly increased the plant height, leaf area and stem biomass of Coix lachryma-jobi L.; the chlorophyll content showed an overall upward trend, and the CAT and POD activities significantly increased; at the same time, the root system was more sensitive to exogenous zinc, and the root length significantly decreased by 28.09%, while the number of tap roots increased by 16.86%; (3) zinc application and nitrogen deficiency significantly increased the plant height and root length of Coix lachryma-jobi L. seedlings by 20.30% and 17.72%, respectively; the antioxidant enzyme CAT activity significantly increased by 171.01%, and there was no significant change in POD activity and MDA accumulation. This study indicates that zinc application under nitrogen stress could promote the growth of Coix lachryma-jobi L. seedlings, balance the seedlings’ antioxidant system and improve their adaptability, thus alleviating the adverse effects caused by nitrogen stress to a certain extent.

Key words: Coix lachryma-jobi L., nitrogen stress, exogenous zinc, seedling growth, antioxidant system

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