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中国农学通报

中国农学通报 ›› 2008, Vol. 24 ›› Issue (4): 85-88.

所属专题： 生物技术

• 生物技术科学 • 上一篇    下一篇

青岛百合组织培养研究

齐春敏,王奎玲,刘庆超,刘庆华   

  • 收稿日期:2008-02-26 修回日期:2080-03-04 出版日期:2008-04-05 发布日期:2008-04-05

Research on Tissue Culture of Lilium tsingtauense Gilg

Qi Chunmin, Wang Kuiling, Liu Qingchao, Liu Qinghua   

  • Received:2008-02-26 Revised:2080-03-04 Online:2008-04-05 Published:2008-04-05

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摘要： 以青岛百合叶片为外植体，研究了消毒时间、培养基、生长调节物质对青岛百合叶片不定芽发生、增殖和生根的影响。结果表明：用0.1%氯化汞对青岛百合叶片消毒5 min时效果最好，污染率为31%，萌动率为79%，诱导率为65.2%。青岛百合最佳叶片不定芽分化培养基为MS+6-BA2.0 mg/L+NAA 0.2mg/L；最佳不定芽增殖培养基为MS+6-BA 1.0 mg/L+NAA 0.2mg/L；最佳生根的培养基为1/2MS+NAA 0.2 mg/L，形成了较为完善的组织培养再生体系。

关键词: 毛豆, 毛豆, 大豆疫病, 抗性鉴定

Abstract: The leaf of Lilium tsingtauense Gilg. were used as experimental materials. And the effect of different sterilized time, kinds of hormone and its combination on advenitious buds proliferation, plant regeneration and root regeneration were studied the in this paper. The results showed that explant sterilized in 0.1% aqueous mercuric chloride for 5 min had better effect, and the contamination rate was 31%, the germinating rate was 79%, inducement rate was 65.2%. MS+6-BA 2.0 mg/L+NAA 0.2 mg/L was suitable for advenitious buds proliferation from the leaf. The optimum medium was MS+6-BA 1.0mg/L+NAA 0.2mg/L. The best medium for rooting was 1/2MS+NAA 0.2mg/L. The comparatively perfect tissue culture regeneration system was formed.