拟南芥中花特异表达启动子PCHS的分离及其功能初探

摘要/Abstract

摘要： 根据已发表的拟南芥启动子序列（AF248988）设计合成一对引物，以拟南芥（Arabidopsis）基因组DNA为模板，通过PCR技术获得了约含500 bp大小的DNA片段，经纯化后测序得到531 bp的目的片段。通过DNAman进行序列分析表明，与文献报道的PCHS启动子序列有99％的同源，含有四个花特异表达的调控元件。将此启动子替换pBI121上的CaMV35S启动子构建植物表达载体，农杆菌介导法浸染矮牵牛花、茎、叶、根。GUS瞬时表达结果表明，在花上出现较深的蓝色，而在茎上的蓝色很浅，在根和叶中不显蓝色，初步确定该启动子具有较强的花特异表达特性。

关键词: 黄瓜, 黄瓜, 基质用量, 生长发育, 产量

Abstract: A polymerase chain reaction（PCR）amplification was used to isolate a flower-specific expression promoter PCHS from Arabidopsis genomic DNA. Sequence analysis revealed that PCHS was 531 bp in total length and shared significant nucleotides identity 99% with GenBank databases, which contained four flower-specific expression regulatory elements. Intermediate vector pPCHS was constructed by inserting the promoter to the upstream of GUS in pB1121, where the original CaMV35S promoter was replaced by the PCHS promoter. The flower, stem, leaf ,and root of petunia hybrida were transformed via Agrobacterium tumefaciens. Histochemical localization of GUS activity indicated that the PCHS promoter could confer a flower-specific expression pattern to GUS.

李刚,陶妹英,贾彩红,张建斌,徐碧玉,金志强. 拟南芥中花特异表达启动子PCHS的分离及其功能初探[J]. 中国农学通报, 2008, 24(4): 89-93.

Li Gang, Tao Meiying, Jia Caihong,Zhang Jianbin, Xu Biyu Jin Zhiqiang. Cloning and Function Analysis of a Flower-specific Expression Promoter PCHS[J]. Chinese Agricultural Science Bulletin, 2008, 24(4): 89-93.

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