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中国农学通报

中国农学通报 ›› 2008, Vol. 24 ›› Issue (4): 94-98.

所属专题： 生物技术； 小麦

• 生物技术科学 • 上一篇    下一篇

小麦GAPDH 基因克隆及序列分析

岳彩凤,康国章,刘 超,郭天财,朱云集,沈丙权   

  • 收稿日期:2008-01-17 修回日期:2008-01-23 出版日期:2008-04-05 发布日期:2008-04-05

Cloning and Sequencing of GAPDH Gene in Common Wheat(Tritucum aestivum L.)

Yue Caifeng, Kang Guozhang, Liu Chao, Guo Tiancai, Zhu Yunji, Shen Bingquan   

  • Received:2008-01-17 Revised:2008-01-23 Online:2008-04-05 Published:2008-04-05

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摘要： 采用Trizol法提取高质量小麦总RNA，并运用RT-PCR方法克隆了小麦GAPDH （甘油醛三磷酸脱氢酶）基因的部分序列(EU022331)，长度为604 bp，核苷酸序列比对结果表明，该序列与大麦(M36650)、玉米(AY109397)、番茄(BT012693)GAPDH核苷酸序列的同源性分别为95%、86%和84%。GAPDH基因的克隆为在禾谷类作物研究的应用提供了一个较好的对照基因。

关键词: 土地生态环境, 土地生态环境, 指标体系, 生态环境区划, 评价

Abstract: The total RNA of wheat leaves was extracted with Trizol, and the partial cDNA sequence of wheat GAPDH gene(EU022331) was amplified through RT-PCR. The colned GAPDH gene (604 bp) was 95 %, 86 % and 84% identified with to M36650(Barley), AY109397 (maize), BT012693(tomato) , respectively. This partial sequence provided a good background on studying expression of many cereal genes.