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中国农学通报

中国农学通报 ›› 2008, Vol. 24 ›› Issue (4): 99-103.

所属专题： 生物技术

• 生物技术科学 • 上一篇    下一篇

黄秋葵基因组DNA提取及鉴定

黄 捷, ,陈晓斌,叶花兰,刘国道,   

  • 修回日期:1900-01-01 出版日期:2008-04-05 发布日期:2008-04-05

The Extraction and Identification of Genomic DNA from Okra

Huang Jie, , Chen Xiaobin, Ye Hualan, Liu Guodao,   

  • Revised:1900-01-01 Online:2008-04-05 Published:2008-04-05

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摘要： 黄秋葵细胞内含有丰富的果胶类多糖等次生物质，降低了从中提取总DNA的产量和质量。为了获得高质量的基因组DNA，采用改进的十六烷基三甲基溴化铵（CTAB）法进行提取，并对总DNA进行了纯度和浓度的鉴定。结果表明，改良的CTAB法可有效去除次生物质对DNA的干扰，降低DNA的粘稠度，样品DNA的质量和纯度较高，可用于随机扩增多态性DNA（RAPD）扩增等分子标记分析。

关键词: 污泥膨胀, 污泥膨胀, 丝状菌膨胀, 影响因素, 控制措施

Abstract: The content of pectic polysaccharides and other secondary metabolites is high in cell of Okra, which influences frequently the yield and quality of genome DNA when being extracted. In this study, a new method of CTAB was introduced which do best for characterization the purity and concentration of genome DNA. The result showed that it could effectively eliminate the affections of the secondary materials to extracted genome DNA from Okra, and which be suitable for RAPD analysis and other molecular maker.