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中国农学通报 ›› 2009, Vol. 25 ›› Issue (18): 338-334.

所属专题: 玉米

• 植物保护 农药 • 上一篇    下一篇

玉米弯孢叶斑病菌致病性分化及几种鉴定技术比较

鄢洪海   

  • 收稿日期:2009-04-07 修回日期:2009-06-29 出版日期:2009-09-20 发布日期:2009-09-20
  • 基金资助:

    国家“九五”科技攻关项目;青岛市自然科学基金项目

Pathogenic differentiation and identification technique of Curvularia lunata on Maize

  • Received:2009-04-07 Revised:2009-06-29 Online:2009-09-20 Published:2009-09-20

摘要:

本研究从我国20余个主栽玉米品种和20个骨干自交系中筛选鉴别寄主,并辅助采用RAPD和同工酶电泳的遗传和生理生化分析,对现阶段我国玉米弯孢叶斑病菌致病性分化现状进行了测定。研究表明:自交系沈135、78599-1、 Mo17、477、C8605、E28、7922和黄早4具有较强的鉴别特性,通过8个鉴别寄主将采自我国玉米产区的23株弯孢叶斑病菌分为6个致病类型,其中类型A致病性强、分布广,为优势致病类群;类型D主要分布在我国零星发生的地区,为弱致病类群。不同方法鉴定的结果具有相关性,特别是对那些致病性分化明显、致病性强的菌株三种鉴定方法鉴定结果关系密切,都能对应地划分在同一个组里。

关键词: 亚麻籽, 亚麻籽, 溶剂萃取, 微波烘烤, 水煮, 氰化氢

Abstract:

As the differential hosts, Shen135,78599-1、Mo17、477、C8605、E28、7922 were selected to studied by physiology and biochemistry, isozyme analysis of hereditary and RAPD from more than 20 main culture varieties and 20 skeleton inbred lines. Pathogenic differentiation were determined for Curvularia lunata on Maize at present. The result indicated that physiological differentiation were consistent between seedling stage identification in greenhouse and adult plant in field. Seedling stage identification had the features of simple operation and determining conditions were easily controlled. Based on 8 differential hosts from maize growing area, there were 6 pathogenic types in 20 Curvularia lunata strains. The type A was superior pathogenic group which showed high pathogenicity and wide distribution, however, type D was inferior one spreading small range. The result of different methods had correlation, especially for high pathogenic differentiation strains. All can be divided into the corresponding group in the same. 98-18,98-11 albino strains are divided in one group. with other strains in the pathogenicity, aspects of phenotypic differences.