快速溶剂萃取技术在2种食药用真菌多糖提取中的应用

doi:10.11924/j.issn.1000-6850.casb2020-0429

中国农学通报 ›› 2021, Vol. 37 ›› Issue (16): 150-155.doi: 10.11924/j.issn.1000-6850.casb2020-0429

所属专题：生物技术；园艺

• 食品·营养·检测·安全 • 上一篇下一篇

快速溶剂萃取技术在2种食药用真菌多糖提取中的应用

闫佳佳¹^,²(), 万璐¹^,², 吴桐¹^,³(), 郑春英¹^,²()

¹黑龙江大学农业微生物技术教育部工程研究中心,哈尔滨 150500
²黑龙江大学生命科学学院/黑龙江省普通高校微生物重点实验室,哈尔滨 150080
³黑龙江大学生命科学学院/黑龙江省普通高校分子生物学重点实验室,哈尔滨 150080

收稿日期:2020-09-07 修回日期:2021-01-18 出版日期:2021-06-05 发布日期:2021-06-16
通讯作者: 吴桐,郑春英
作者简介:闫佳佳,女,1996年出生,黑龙江齐齐哈尔人,硕士研究生,研究方向为食品和药物生物活性挖掘及研发。通信地址：150080 黑龙江省哈尔滨市南岗区学府路74号黑龙江大学生命科学学院,Tel：0451-86608586,E-mail： 2424643682@qq.com。
基金资助:
黑龙江大学校企合作横向项目“一株产原儿茶酸的枯草芽孢杆菌”(20170223)

Application of Pressurized Solvent Extraction Technology in the Extraction of Polysaccharide from Two Kinds of Edible and Medicinal Fungi

Yan Jiajia¹^,²(), Wan Lu¹^,², Wu Tong¹^,³(), Zheng Chunying¹^,²()

¹Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin 150500
²Key Laboratory of Microbiology, College of Heilongjiang Province/ School of Life Sciences, Heilongjiang University, Harbin 150080
³Key Laboratory of Molecular Biology, College of Heilongjiang Province/ School of Life Sciences, Heilongjiang University, Harbin 150080

Received:2020-09-07 Revised:2021-01-18 Online:2021-06-05 Published:2021-06-16
Contact: Wu Tong,Zheng Chunying

摘要/Abstract

摘要：

为建立快速溶剂萃取技术提取食药用真菌多糖的新方法,以猪苓、茯苓为研究对象,采用单因素及正交实验优选快速溶剂萃取法提取多糖的最佳工艺条件。结果表明,快速溶剂萃取法用于猪苓多糖提取的最佳工艺参数为提取温度110℃,提取压力110 bar,提取时间20 min,循环2次,在此条件下,猪苓多糖的含量为(7.8684±0.1096) mg/g。快速溶剂萃取法提取茯苓多糖的最佳工艺参数是提取温度110℃,提取压力120 bar,提取时间25 min,循环2次,在此条件下,茯苓多糖的含量为(8.6965±0.1087) mg/g。快速溶剂萃取技术能够快速、准确地适用于2种食药用真菌多糖的提取。

关键词: 快速溶剂萃取法, 猪苓多糖, 茯苓多糖, 提取工艺, 单因素实验, 正交实验

Abstract:

The paper aims to develop Pressurized Solvent Extraction (PSE) for extracting polysaccharides in two kinds of edible and medicinal fungi with the Polyporus umbellatus and Poria cocos were chosen as raw materials, the single factor and orthogonal experiments were used to explore the optimal conditions for the extraction of polysaccharide by PSE. The results showed that the optimized parameters of PSE employed temperature 110℃, pressure 110 bar, and extracting time was set at 20 min for 2 times to extract polysaccharides in Polyporus umbellatus completely. Under the condition, the content of Polyporus umbellatus polysaccharide was (7.8684±0.1096) mg/g. Meanwhile, the optimized parameters of PSE employed temperature 110℃, pressure 120 bar, and extracting time was set at 25 min for 2 times to extract Poria cocos polysaccharides completely. Under the condition, the content of Poria cocos polysaccharide was (8.6965±0.1087) mg/g. As a conclusion, PSE can be used to extract polysaccharides from the two kinds of edible and medicinal fungi quickly and accurately.

Key words: PSE, Polyporus umbellatus polysaccharides, Poria cocos polysaccharides, extraction technology, single factor experiment, orthogonal experiment

中图分类号:

R931

闫佳佳, 万璐, 吴桐, 郑春英. 快速溶剂萃取技术在2种食药用真菌多糖提取中的应用[J]. 中国农学通报, 2021, 37(16): 150-155.

Yan Jiajia, Wan Lu, Wu Tong, Zheng Chunying. Application of Pressurized Solvent Extraction Technology in the Extraction of Polysaccharide from Two Kinds of Edible and Medicinal Fungi[J]. Chinese Agricultural Science Bulletin, 2021, 37(16): 150-155.

图/表 8

参考文献 38

[1]	Ramunė B, Paulius K, Laura T, et al. Recovery of bioactive substances from rowanberry pomace by consecutive extraction with supercritical carbon dioxide and pressurized solvents[J]. Journal of Industrial and Engineering Chemistry, 2020,85.
[2]	Ester H C, Merichel P, María L M, et al. Sustainable extraction of proteins and bioactive substances from pomegranate peel (Punica granatum L.) using pressurized liquids and deep eutectic solvents[J]. Innovative Food Science and Emerging Technologies, 2020,60.
[3]	Li J, Zhang S, Zhang M, et al. Novel approach for extraction of grape skin antioxidants by accelerated solvent extraction: Box-Behnken design optimization[J]. Journal of Food Science and Technology, 2019,56(11):223-225.
[4]	Šeregelj V, Tumbas S, Vesna M, et al. Accelerated solvent extraction of bioactive compounds from carrot: Optimization of response surface methodology[J]. Journal of the Serbian Chemical Society, 2018,83(11):231-234.
[5]	Pimentel M S, Borrás L I, Lozano S J, et al. Pressurized GRAS solvents for the green extraction of phenolic compounds from Hibiscus sabdariffa calyces[J]. Food Research International, 2020,137:345-347.
[6]	Yoong K L, Yang F C, Chang J S. Extraction of polysaccharides from edible mushrooms: Emerging technologies and recent advances[J]. Carbohydrate Polymers, 2020,117006.
[7]	国家药典委员会. 中华人民共和国药典2020年版(一部)[M]. 北京: 中国医药科技出版社, 2020: 331.
[8]	Zhao Y Y. Traditional uses, phytochemistry, pharmacology, pharmacokinetics and quality control of Polyporus umbellatus (Pers.) Fries: A review[J]. Journal of Ethnopharmacology, 2013,149(1):342-345.
[9]	Song R Q, Nan T G, Yang Y, et al. Study on polysaccharide content and monosaccharide composition of Polyporus umbellatus from different production areas[J]. China journal of Chinese Materia Medica, 2019,44(17):354-357.
[10]	Liu J, Zhou J, Zhang Q Q, et al. Monosaccharide analysis and fingerprinting identification of polysaccharides from Poria cocos and Polyporus umbellatus by HPLC combined with chemometrics methods[J]. Chinese Herbal Medicines, 2019,11(4):406-411. doi: 10.1016/j.chmed.2019.05.008 URL
[11]	Chen X D, Chong Y K, Zhang W W, et al. Discrimination of authentic Polyporus umbellatus and counterfeit by Fourier Transform Infrared and two dimensional infrared correlation spectroscopy[J]. Journal of Molecular Structure, 2020,1199.
[12]	Kunca V, Pavlik M. Fruiting Body Production of, and Suitable Environmental Ranges for, Growing the Umbrella Polypore Medicinal Mushroom, Polyporus umbellatus (Agaricomycetes), in Natural Conditions in Central Europe[J]. International Journal of Medicinal Mushrooms, 2019,21(2):435-439.
[13]	Zhao Y Y, Xie R M, Chao X, et al. Bioactivity-directed isolation, identification of diuretic compounds from Polyporus umbellatus[J]. Eth- nopharmacol, 2009,126:184-187.
[14]	Guo Z H, Zang Y J, Zhang L J, et al. The efficacy of Polyporus umbellatus polysaccharide in treating hepatitis B in China[J]. Progress in Molecular Biology & Translational Science, 2019,163:329-360.
[15]	Li H L, Yan Z, Xiong Q P, et al. Renoprotective effect and mechanism of polysaccharide from Polyporus umbellatus sclerotia on renal fibrosis[J]. Carbohydrate Polymers, 2019,212:342-345.
[16]	Xing X K, Men J X, Song L L, et al. Do the Main Components of the Sclerotia of Umbrella Polypore Mushroom, Polyporus umbellatus (Agaricomycetes), Correlate with Armillaria Associates[J]. International Journal of Medicinal Mushrooms, 2020,22(5):57-59.
[17]	Liu G K, Li N, Zhang Y J, et al. LC/MS fingerprint and simultaneous quantification of main bioactive compounds in Polyporus umbellatus (Pers.) Fr. from different regions and developmental stages[J]. Microchemical Journal, 2018,15:97-101. doi: 10.1016/0026-265X(70)90170-0 URL
[18]	Xiong G, Li X F, Mu J J, et al. Preparation, physicochemical characterization, and anti-proliferation of selenium nanoparticles stabilized by Polyporus umbellatus polysaccharide[J]. International Journal of Biological Macromolecules, 2020,152.
[19]	Zhao Y Y, Feng Y L, Du X, et al. Diuretic activity of the ethanol and aqueous extracts of the surface layer of Poria cocos in rat[J]. Ethnopharmacol, 2012,144:775-778. doi: 10.1016/j.jep.2012.09.033 URL
[20]	Lee H C, Cheng W Y, Huang B E, et al. Huang Anti-inflammatory and hypoglycemic efficacy of Poria cocos and Dioscorea opposita in prediabetes mellitus rats[J]. RSC Adv, 2014,4:55649-55657. doi: 10.1039/C4RA10539G URL
[21]	Feng Y L, Lei P, Tian T, et al. Diuretic activity of some fractions of the epidermis of Poria cocos[J]. Journal of Ethnopharmacology, 2013,150(3):3425-3427.
[22]	Zhou L, Zhang Y, Gapte L A, et al. Ng Cytotoxic and anti-oxidant activities of lanostane-type triterpenes isolated from Poria cocos[J]. Chem Pharm Bull, 2008,56:1459-1462. doi: 10.1248/cpb.56.1459 URL
[23]	Lin T Y, Lu M K, Chang C C. Structural identification of a fucose-containing 1,3--mannoglucan from Poria cocos and its anti-lung cancer CL1-5 cells migration via inhibition of TGF R-mediated signaling[J]. International Journal of Biological Macromolecules, 2020,157:311-318. doi: 10.1016/j.ijbiomac.2020.04.014 URL
[24]	Ríos J L. Chemical Constituents and Pharmacological Properties of Poria cocos[J]. Planta Med, 2011,77(7):56-59.
[25]	Jia X J, Ma L S, Li P, et al. Prospects of Poria cocos polysaccharides: Isolation process, structural features and bioactivities[J]. Trends in Food Science & Technology, 2016,54:2-62.
[26]	Esteban C I. Medicinal interest of Poria cocos (= Wolfiporia extensa)[J]. Revista Iberoamericana de Micología, 2009,26(2):103-107. doi: 10.1016/S1130-1406(09)70019-1 URL
[27]	Zhu L, Xu J, Wang R, et al. Correlation between quality and geographical origins of Poria cocos revealed by qualitative fingerprint profiling and quantitative determination of triterpenoid acids[J]. Molecules, 2018,23:22009.
[28]	Yang P F, Tao H, Dong W, et al. Phytochemical and chemotaxonomic study of Poria cocos (Schw.) Wolf[J]. Biochemical Systematics and Ecology, 2019,83.
[29]	He P F, Zhang A Q, Zhang F M. Linhardt, Peilong Sun, Structure and bioactivity of a polysaccharide containing uronic acid from Polyporus umbellatus sclerotias[J]. Carbohydrate Polymers, 2016,152:222-230. doi: 10.1016/j.carbpol.2016.07.010 URL
[30]	Li W F, Zhao J M, Yao Q, et al. Polysaccharides from Poria cocos (PCP) inhibits ox-LDL-induced vascular smooth muscle cells proliferation and migration by suppressing TLR4/NF-κB p65 signaling pathway[J]. Journal of Functional Foods, 2019,60:103391. doi: 10.1016/j.jff.2019.05.047 URL
[31]	Feng Y N, Zhang X F. Polysaccharide extracted from Huperzia serrata using response surface methodology and its biological activity[J]. International Journal of Biological Macromolecules, 2020,157.
[32]	Yang X S, Fang C, Huang G L. Extraction and analysis of polysaccharide from Momordica charantia[J]. Industrial Crops & Products, 2020,153.
[33]	吴桐, 徐慧春, 郑春英, 等. 快速溶剂萃取法提取刺五加叶中的黄酮类成分[J]. 中国食品学报, 2013,13(7):59-65.
[34]	Lin L, Wang Y, Wang F X, et al. Determination of polysaccharides content of Gentiana farreri from different producing areas based on anthrone-sulfuric acid method[J]. China Journal of Chinese Materia Medica, 2014,39(14):345-348.
[35]	牛雯颖. 几种常见药用真菌糖类成分分析[D]. 哈尔滨:黑龙江大学, 2008.
[36]	Rizwan A, Niyaz A, Sadeq A, et al. Green accelerated solvent extraction (ASE) with solvent and temperature effect and green UHPLC-DAD analysis of Phenolics in Pepper fruit (Capsicum annum L.)[J]. Food Composition and Analysis, 2020,103:245-249.
[37]	Niyaz A, Rizwan A, Wejdan S, et al. Solvent and temperature effect of accelerated solvent extraction (ASE) coupled with ultra-high-pressure liquid chromatography (UHPLC-PDA) for the determination of methyl xanthines in commercial tea and coffee[J]. Food Chemistry, 2020,311:534-540.
[38]	王宇晴, 牛雯颖, 郑春英, 等. 快速溶剂萃取法在灵芝多糖提取中的应用[J]. 中国调味品, 2020,45(2):107-111.

组别		因素				猪苓多糖/(mg/g)	茯苓多糖/(mg/g)
组别		A.PSE温度	B.PSE压力	C.PSE时间	D	猪苓多糖/(mg/g)	茯苓多糖/(mg/g)
1		1	1	1	1	4.1521	5.4213
2		1	2	2	2	3.1626	6.3118
3		1	3	3	3	3.3846	6.8119
4		2	1	2	3	5.9104	5.6779
5		2	2	3	1	4.4972	5.8444
6		2	3	1	2	4.2856	6.5893
7		3	1	3	2	5.8114	8.0469
8		3	2	1	3	6.2440	6.0326
9		3	3	2	1	7.5581	8.4141
猪苓多糖	K₁	10.6993	15.8739	14.6817		ΣY=45.0060 CT=225.0600
	K₂	14.6932	13.9038	16.6311
	K₃	19.6135	15.2283	13.6932
	R	715.0545	677.1975	679.6495
	S	13.2915	0.6725	1.4898
茯苓多糖	K₁	18.5450	19.1461	18.0432		ΣY=59.1502 CT=388.7496
	K₂	18.1116	18.1888	20.4038
	K₃	22.4936	21.8153	20.7032
	R	1177.9091	1173.3128	1170.4947
	S	3.8868	2.3547	1.4153

组别		因素				猪苓多糖/(mg/g)	茯苓多糖/(mg/g)
组别		A.PSE温度	B.PSE压力	C.PSE时间	D	猪苓多糖/(mg/g)	茯苓多糖/(mg/g)
1		1	1	1	1	4.1521	5.4213
2		1	2	2	2	3.1626	6.3118
3		1	3	3	3	3.3846	6.8119
4		2	1	2	3	5.9104	5.6779
5		2	2	3	1	4.4972	5.8444
6		2	3	1	2	4.2856	6.5893
7		3	1	3	2	5.8114	8.0469
8		3	2	1	3	6.2440	6.0326
9		3	3	2	1	7.5581	8.4141
猪苓多糖	K₁	10.6993	15.8739	14.6817		ΣY=45.0060 CT=225.0600
	K₂	14.6932	13.9038	16.6311
	K₃	19.6135	15.2283	13.6932
	R	715.0545	677.1975	679.6495
	S	13.2915	0.6725	1.4898
茯苓多糖	K₁	18.5450	19.1461	18.0432		ΣY=59.1502 CT=388.7496
	K₂	18.1116	18.1888	20.4038
	K₃	22.4936	21.8153	20.7032
	R	1177.9091	1173.3128	1170.4947
	S	3.8868	2.3547	1.4153

成分	方差来源	离差平方和	自由度	均方	F值	显著性
猪苓多糖	A	13.2915	2	6.6458	177.6952	<0.01
	B	0.6725	2	0.3363	9.0000	>0.05
	C	1.4898	2	0.7449	19.9171	<0.05
	e	0.0747	2	0.0374
茯苓多糖	A	3.8868	2	1.9434	24.6938	<0.05
	B	2.3547	2	1.1774	14.9606	>0.05
	C	1.4153	2	0.7077	9.0000	>0.05
	e	0.1573	2	0.0787

成分	方差来源	离差平方和	自由度	均方	F值	显著性
猪苓多糖	A	13.2915	2	6.6458	177.6952	<0.01
	B	0.6725	2	0.3363	9.0000	>0.05
	C	1.4898	2	0.7449	19.9171	<0.05
	e	0.0747	2	0.0374
茯苓多糖	A	3.8868	2	1.9434	24.6938	<0.05
	B	2.3547	2	1.1774	14.9606	>0.05
	C	1.4153	2	0.7077	9.0000	>0.05
	e	0.1573	2	0.0787

方法	猪苓多糖含量	茯苓多糖含量
热水提取法	2.3874±0.1066	3.4476±0.1073
PSE法	7.8684±0.1096	8.6965±0.1087

快速溶剂萃取技术在2种食药用真菌多糖提取中的应用

Application of Pressurized Solvent Extraction Technology in the Extraction of Polysaccharide from Two Kinds of Edible and Medicinal Fungi

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水平	因素
水平	A.PSE温度/℃	B.PSE压力/bar	C.萃取时间/min
1	90	100	15
2	100	110	20
3	110	120	25

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