河南南阳地产绿米稻壳中花青素的提取工艺研究

doi:10.11924/j.issn.1000-6850.casb17050063

中国农学通报 ›› 2018, Vol. 34 ›› Issue (18): 71-76.doi: 10.11924/j.issn.1000-6850.casb17050063

所属专题：水稻

河南南阳地产绿米稻壳中花青素的提取工艺研究

孙美玲,梁月洋,王红朵,张仲鸣

南阳理工学院张仲景国医国药学院,南阳理工学院张仲景国医国药学院,南阳理工学院张仲景国医国药学院,南阳理工学院张仲景国医国药学院

收稿日期:2017-05-15 修回日期:2018-06-06 接受日期:2017-06-23 出版日期:2018-06-26 发布日期:2018-06-26
通讯作者: 张仲鸣
基金资助:
河南省自然科学基金“下丘脑低表达BDNF对Ang II 诱导的高血压致敏的抵抗及其细胞和神经内分泌调节机理”(162300410203)；南阳理工学院特聘教授启动基金“预防高血压和肥胖的植物成分筛选和机理研究”(NIT201401015)。

Extraction Process of Anthocyanins from Husk of Local-grown Rice Oryza Sativa L. Nanyangensis in Nanyang, Henan

Received:2017-05-15 Revised:2018-06-06 Accepted:2017-06-23 Online:2018-06-26 Published:2018-06-26

摘要/Abstract

摘要： 摘要：本研究旨在探索从河南南阳当地培育的一个水稻品种南阳绿米的稻壳中提取花青素的最适条件。利用0.1mol/L的盐酸乙醇连续提取3次，依据所得溶液的530nm、620nm和650nm波长下的光吸收值和Greey公式计算出的花青素含量，分析了乙醇浓度、温度、提取时间和料液比等单因素对提取量的影响；在此基础上，设计了四因素三水平的正交试验，并利用正交分析法确定最优化的提取条件组合。结果表明，最佳提取效果的条件组合为乙醇浓度60%、温度60℃、提取时间为9 h、料液比为1:20，在此条件下，100 g稻壳可提取的花青素量为588.41 mg，由此推测，每公顷南阳绿米的稻壳中可得到约13 kg的花青素。因此，南阳绿米稻壳中的花青素含量值得提取，南阳绿米是一个具有附加经济效益的新品种，值得推广种植。

关键词: 刚毛柽柳, 刚毛柽柳, GRAS 转录因子, 启动子, 瞬时表达

Abstract: This investigation aims to obtain the optimal condition for extracting anthocyanins from husk of a local- grown rice subspecies Oryza Sativa L. Nanyangensis in Nanyang, Henan. After three consecutive extractions using 0.1mol/L HCI acidic ethanol, the absorbance value of anthocyanins were obtained by ultraviolet-visible spectrophotometer at the wavelength of 530nm, 620nm and 650nm, respectively, and the quantity was determined by Greey formula. Based on the quantities of anthocyanins in the extractions, singlefactor effect including alcohol concentration, temperature, incubation time and the ratio of husk to ethanol was evaluated. Then orthogonal experiments of four factors with three levels were designed and conducted to get the optimal condition for anthocyanins extraction. Orthogonal analysis revealed that the optimal condition was 60% ethanol, 9 h, 60℃ , and 1:20 ratio of husk to ethanol (w/v). Under the optimal condition, the yield of anthocyanins from 100g husk was 588.41mg. According to this, the predicted yield of anthocyanins is about 13 kg per hectare. So, O. Sativa L. Nanyangensis has an appreciable amount of anthocyanins in its husk. Therefore, this rice subspecies is of additional value, and is worthy to be popularized in more areas.

孙美玲,梁月洋,王红朵,张仲鸣. 河南南阳地产绿米稻壳中花青素的提取工艺研究[J]. 中国农学通报, 2018, 34(18): 71-76.

参考文献

1. Chen, Y.F., et al., Purple rice anthocyanin extract protects cardiac function in STZ-induced diabetes rat hearts by inhibiting cardiac hypertrophy and fibrosis. J Nutr Biochem, 2016. 31: p. 98-105.
2. Chen, X.Y., et al., Black Rice Anthocyanins Suppress Metastasis of Breast Cancer Cells by Targeting RAS/RAF/MAPK Pathway. Biomed Res Int, 2015. 2015: p. 414250.
3. Suwannakul, N., et al., Purple rice bran extract attenuates the aflatoxin B1-induced initiation stage of hepatocarcinogenesis by alteration of xenobiotic metabolizing enzymes. Asian Pac J Cancer Prev, 2015. 16(8): p. 3371-6.
4. 王倩倩, et al., 云南不同品种有色稻米HPLC指纹图谱的建立及质量评价. 中国农学通报, 2012. 28(12): p. 99-104.
5. David, R.G., Y. Wengui, and J.N. Rutger, Red Rice (Oryza sativa) Plant Types Affect Growth, Coloration, and Flowering Characteristics of First- and Second-Generation Crosses with Rice. Weed Technology, 2006. 20(4): p. 839-852.
6. 王秋霜, et al., 红紫芽茶叶花青素提取分离及pH稳定性初探. 中国农学通报, 2014. 30(9): p. 291-294.
7. G, D.U., et al., Metabolomics of Healthy Berry Fruits. Curr Med Chem, 2016.
8. Du, C., et al., Anthocyanins inhibit high-glucose-induced cholesterol accumulation and inflammation by activating LXRalpha pathway in HK-2 cells. Drug Des Devel Ther, 2015. 9: p. 5099-113.
9. 王振江, et al., 桑椹花青素对大鼠佐剂性关节炎抑制作用. 中国公共卫生, 2009. 25(2): p. 181-183.
10. Thummayot, S., et al., Neuroprotective effect of purple rice extract and its constituent against amyloid beta-induced neuronal cell death in SK-N-SH cells. Neurotoxicology, 2014. 45: p. 149-58.
11. Punvittayagul, C., et al., Mutagenicity and antimutagenicity of hydrophilic and lipophilic extracts of Thai northern purple rice. Asian Pac J Cancer Prev, 2014. 15(21): p. 9517-22.
12. Munagala, R., et al., Exosomal formulation of anthocyanidins against multiple cancer types. Cancer Lett, 2017. 393: p. 94-102.
13. Amin, F.U., et al., Anthocyanins encapsulated by PLGA@PEG nanoparticles potentially improved its free radical scavenging capabilities via p38/JNK pathway against Abeta1-42-induced oxidative stress. J Nanobiotechnology, 2017. 15(1): p. 12.
14. 伍树松, et al., 花青素作为饲料添加剂的应用前景. 动物营养学报, 2011. 23(12): p. 2097-2104.
15. Sui, X., Y. Zhang, and W. Zhou, Bread fortified with anthocyanin-rich extract from black rice as nutraceutical sources: Its quality attributes and in vitro digestibility. Food Chem, 2016. 196: p. 910-6.
16. Cassidy, A., et al., Intake of dietary flavonoids and risk of epithelial ovarian cancer. Am J Clin Nutr, 2014. 100(5): p. 1344-51.
17. Niziol-Lukaszewska, Z., et al., Hydrophilic Dogwood Extracts as Materials for Reducing the Skin Irritation Potential of Body Wash Cosmetics. Molecules, 2017. 22(2).
18. Beidokhti, M.N. and A.K. Jager, Review of Antidiabetic Fruits, Vegetables, Beverages, Oils and Spices commonly consumed in the Diet. J Ethnopharmacol, 2017.
19. 刘仁道, 张. 猛, and 李新贤, 草莓和蓝莓果实花青素提取及定量方法的比较. 园艺学报, 2008. 35(5): p. 655-660.

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河南南阳地产绿米稻壳中花青素的提取工艺研究

Extraction Process of Anthocyanins from Husk of Local-grown Rice Oryza Sativa L. Nanyangensis in Nanyang, Henan

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