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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (18): 364-366.

• 植物保护 农药 • 上一篇    下一篇

氯氰菊酯降解菌Y-3的诱变育种与筛选

洪永聪   

  • 收稿日期:2009-05-06 修回日期:2009-05-22 出版日期:2009-09-20 发布日期:2009-09-20
  • 基金资助:

    杀虫防病工程内生细菌植物疫苗研究;蔬菜氯氰菊酯农药微生物降解研究

Selection by Mutation of Cypermethrin Degrading Strain Y-3

  • Received:2009-05-06 Revised:2009-05-22 Online:2009-09-20 Published:2009-09-20

PDF (PC)

513

摘要：

以从菠菜叶片内分离到对氯氰菊酯降解率较高的菌株Y-3（Corynebacterium vitarumen）为出发菌，进行化学诱变和紫外诱变。结果显示，DES对菌株Y-3的最佳诱变条件为2% 60min、3% 40min和4% 20min，紫外线对对菌株Y-3的最佳诱变条件为5cm 8min、10cm 20min和15cm 30min。通过诱变与筛选，最终获得13株突变株，与野生菌株对比，7株突变株对氯氰菊酯的降解率有明显的提高，其中有3株突变株具有一定的遗传稳定性。

关键词: 猪舍, 猪舍, 保温方式, 经济性

Abstract:

Cypermethrin degrading strain Y-3 (Corynebacterium vitarumen) was used as the original strain, which was isolated from spinach leaves. The result indicated that its optimal condition induced by DES should be 2% 60min, 3% 40min and 4% 20min. The optimal condition of strain Y-3 induced by UV light was tested to be 5cm 8min, 10cm 20min and 15cm 30min. Thirteen mutants were obtained, among which seven mutants were proved to degrade cypermethrin on a higher level, compared with their original strain. Three of those mutants were tested to have hereditary stability to degrade cypermethrin.