欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (22): 0-0.

所属专题： 生物技术； 水产渔业

• 水产 渔业 •    

长江下游铜鱼线粒体DNA（mtDNA）遗传多样性的PCR-RFLP分析

张敏莹   

  • 收稿日期:2009-06-11 修回日期:2009-07-15 出版日期:2009-11-20 发布日期:2009-11-20
  • 基金资助:
    国家科技基础条件平台项目;天津市农业科技成果转化与推广项目

Analysis on Genetic Diversity of mtDNA of Coreius heterodon in the Lower Reaches of the Yangtze River by PCR-RFLP

  • Received:2009-06-11 Revised:2009-07-15 Online:2009-11-20 Published:2009-11-20

PDF (PC)

1253

摘要： 对长江下游铜鱼线粒体DNA 控制区(D-Loop区)和细胞色素b(Cytb)基因进行PCR扩增，扩增片段用10种限制性内切酶酶切。PCR-RFLP研究结果表明， D-Loop区和Cytb基因均未表现出个体之间的长度变异现象。10种核酸内切限制酶中，有 2种对Cytb基因有酶切位点，有3种对D-Loop区有酶切位点。有酶切位点的5种限制性内切酶共检测到3种线粒体DNA单倍型。在Cytb基因检测到铜鱼个体间的变异，在D-Loop区没有检测到铜鱼个体间的变异。长江下游铜鱼线粒体DNA单倍型多样性指数（h）为0.6071，线粒体DNA遗传多样性较低。

关键词: 母猪, 母猪, 诱导发情, 发情率, 受胎率, 排卵

Abstract: Mitochondria1 control （D-Loop ）region and cytochrome b (Cyt b) gene of Bronze gudgeon (Coreius heterodon) in the lower reaches of the Yangtze River were cloned by PCR . Cloned fragments were digested by ten kinds of restriction endonucleases . Result of PCR-RFLP showed that no length diversity among different individual were detected both in D-Loop region and Cyt b gene. Among ten restriction endonucleases, Cyt b gene could be digested by two kinds of those and D-Loop region could be digested by three kinds of those. Three kinds of haplotypes were detected with five enzymes. Diversity among individuals of Bronze gudgeon was detected in Cyt b gene , while no diversity was found among different individuals in D-Loop region. The haplotype diversity index of of Bronze gudgeon in the lower reaches of the Yangtze River was 0.6071,which revealed a lower genetic diversity in mtDNA.