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中国农学通报

中国农学通报 ›› 2022, Vol. 38 ›› Issue (35): 90-96.doi: 10.11924/j.issn.1000-6850.casb2021-0518

所属专题： 生物技术； 水产渔业

• 畜牧·动物医学·蚕·蜂 • 上一篇    下一篇

戴瑞奶绵羊血清中生殖激素含量与超排效果相关性研究

郭玉林1(), 张立果2, 郑重1, 王春伟1, 徐全忠1, 武云喜1, 李光鹏1, 苏小虎1, 张立1()   

  1. 1内蒙古大学省部共建草原家畜生殖调控与繁育国家重点实验室，呼和浩特 010020
    2乌兰察布市家畜改良工作站，内蒙古乌兰察布 012200
  • 收稿日期:2021-05-18 修回日期:2021-07-01 出版日期:2022-12-15 发布日期:2022-12-10
  • 通讯作者: 张立
  • 作者简介:郭玉林，男，1995年出生，内蒙古赤峰人，硕士研究生，主要从事动物繁育生物技术研究。通信地址：010020 内蒙古呼和浩特内蒙古大学南校区动物中心，E-mail：754868422@qq.com。
  • 基金资助:
    内蒙古自治区科技重大专项“奶绵羊新品种培育及产业化示范”(30900-5173910);内蒙古大学省部共建草原家畜生殖调控与繁育国家重点实验室自主课题“奶用奶绵羊新品种培育绵羊新品种培育与高效繁育技术体系”(SKL-IT-201811)

Study on the Correlation Between Serum Reproductive Hormone Content and Superovulation Effect in Dairy Meade Sheep

GUO Yulin1(), ZHANG Liguo2, ZHENG Zhong1, WANG Chunwei1, XU Quanzhong1, WU Yunxi1, LI Guangpeng1, SU Xiaohu1, ZHANG Li1()   

  1. 1State Key Laboratory of Grassland Livestock Reproductive Regulation and Breeding Jointly Established by the Province and Ministry of Inner Mongolia University, Hohhot 010020
    2Ulanqab City Livestock Improvement Work Station, Ulanqab, Inner Mongolia 012200
  • Received:2021-05-18 Revised:2021-07-01 Online:2022-12-15 Published:2022-12-10
  • Contact: ZHANG Li

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摘要：

目前家畜超数排卵还存在个体差异大、整体效果不稳定的问题。本研究旨在探讨戴瑞奶绵羊血清中生殖激素与供体超排效果的相关性，以期为供体超排性能预测提供依据。本研究分别在超数排卵处理前与配种前采集外周血进行促卵泡生成素(Follicle-stimulating hormone, FSH)、促黄体生成素(Luteinizing hormone, LH)、雌二醇(Estradiol, E2)、孕酮(Progesterone, P4)、抗缪勒管激素(anti-Müllerian hormone, AMH)和抑制素(Inhibin, INH)激素浓度检测，与回收总胚数和可用胚数进行相关性分析。超数排卵前血液检测结果显示：FSH、E2、AMH(FSH: r=0.91, P<0.05; E2: r=0.76, P<0.05; AMH: r=0.86, P<0.05)与回收总胚数和可用胚数呈显著正相关；LH、P4、INH(LH: r=-0.81, P<0.01; P4: r=-0.79, P<0.05; INH: r=-0.56, P<0.05)与回收总胚数和可用胚数呈显著负相关。超数排卵后配种前血液检测结果显示：FSH、E2、P4、AMH(FSH: r=0.62, P<0.05; E2: r=0.93, P<0.05; P4: r=0.69, P<0.05; AMH: r=0.97, P<0.01)与回收总胚数和可用胚数呈显著正相关；LH、INH(LH: r=-0.86, P<0.01; INH: r=-0.60, P<0.05)与回收总数和可用胚数呈显著负相关。本研究结果表明：在超数排卵前戴瑞奶绵羊静脉血液中FSH、LH、E2、P4、AMH、INH这几种激素均与最终超排效果存在相关性。超数排卵前静脉血液中FSH、AMH激素浓度与总胚数和可用胚数拟合度最高，存在高度相关性，因此可作为戴瑞奶绵羊超排性能的检测指标。

关键词: 戴瑞奶绵羊, 超数排卵, 生殖激素, 相关性

Abstract:

At present, superovulation of livestock still has the problems of large individual differences and unstable overall effect. The objective of this study is to explore the correlation between the reproductive hormones in serum of dairy Meade sheep and the superovulation effect of the donor, in order to provide a basis for the prediction of the superovulation performance of the donor. In this study, peripheral blood was collected to determine follicle-stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2), progesterone (P4), anti-Müllerian hormone (AMH) and inhibin (INH) hormone concentrations before superovulation treatment and before mating, and to analyzed their correlations with the total number of embryos recovered and the number of available embryos. The results of blood test before superovulation showed that FSH, E2 and AMH (FSH: r=0.91, P<0.05; E2: r=0.76, P<0.05; AMH: r=0.86, P<0.05;) had a significantly positive correlation with the total number of embryos recovered and the number of available embryos; LH, P4 and INH (LH: r=-0.81, P<0.01; P4: r=-0.79, P<0.05; INH: r=-0.56, P<0.05;) had a significantly negative correlation with the total number of embryos recovered and the number of available embryos. The results of blood test before mating after superovulation showed that FSH, E2, P4 and AMH (FSH r=0.62, P<0.05; E2: r=0.93, P<0.05; P4: r=0.69, P<0.05; AMH: r=0.97, P<0.01) had a significantly positive correlation with the total number of embryos recovered and the number of available embryos; LH and INH (LH: r=-0.86, P<0.01; INH: r=-0.60, P<0.05) had a significantly negative correlation with the total number of embryos recovered and the number of available embryos. The conclusion of this study indicates that FSH, LH, E2, P4, AMH and INH in the venous blood of dairy Meade sheep before superovulation are all related to the final superovulation effect. The FSH and AMH hormone concentrations in the venous blood before superovulation have the highest fit with the total number of embryos and the number of available embryos, and there is a high correlation. Therefore, FSH and AMH can be used as indicators for the detection.

Key words: dairy Meade sheep, superovulation, reproductive hormones, correlation

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