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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (3): 11-14.

所属专题： 生物技术； 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

不同强度光照对母鸡中脑和间脑c-fos基因表达的影响

兰晓宇,胡满,程金金,张毅,胥佳利   

  • 收稿日期:2009-09-22 修回日期:2009-10-21 出版日期:2010-02-05 发布日期:2010-02-05
  • 基金资助:

    国家自然科学基金

Influence on the expression of c-fos gene by the different intensity light stimulation in hen mesencephalon and diencephalons

  • Received:2009-09-22 Revised:2009-10-21 Online:2010-02-05 Published:2010-02-05

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摘要：

为进一步理解光照对母鸡产蛋性能的影响机制，本文用c-fos法探讨了母鸡对不同强度光照刺激的反应。将150日龄的母鸡右眼遮光7d后接受不同强度光照刺激1.5h，暗适应1.5h后灌流固定，取脑制作石蜡切片，采用免疫组化方法检测c-fos基因在中脑和间脑的表达。结果显示：间脑和中脑内有大量Fos样免疫反应阳性神经元，主要见于左侧，分布于视顶盖中央灰质层（SGC），圆核（ROT），外侧膝状腹侧核( Glv)，半月核（SLu），峡核大细胞部(Imc)，狭核小细胞部(Ipc) ，室旁核(PVN)，弓状核(ARC)，动眼神经核（OMv）。不同强度光照诱导母鸡c-fos基因的表达不同，在 SGC ，ROT，SLu，Imc ，Ipc，Glv，20Lux，30Lux组的表达较对照组明显增加，40Lux组次之，10Lux组最低；PVN，ARC为20Lux组表达最强， 30Lux，40Lux组逐渐减弱；而OMv的表达随着强度的增加而加强。表明母鸡对不同强度光照的刺激反应不同。

关键词: 创意农业, 创意农业, 发展路径, 政府

Abstract:

In order to further know the mechanism in the effect of light on egg-laying in hen, the response of hen brain on different light intensity was investigated using c-fos method. The right eyes of 150 days old hens were weared a blinder for 7 days, and received the different intensity light stimulation for 1.5 hours and dark adaptation 1.5 hours. After perfusion with fixative, their brains were removed from the skulls and were made into continuous paraffin sections. Using immunohistochemical method, expression of c-fos gene in mesencephalon and diencephalons was examined. The result showed that a number of Fos-like immunoreactive positive neurons were observed in hen mesencephalon and diencephalons after a light stimulation, being mainly present in left stratum griseum centrale (SGC) of tectum, nucleus rotundus (ROT), nucleus geniculatus lateralis pars ventralis (GLV), nucleus semilunaris (SLu), isthmi pars principais (Imc, Ipc), paraventricular nucleus (PVN), arcuate nucleus (ARC), and nucleus nervi oculomotorii (OMv). The expression of c-fos gene was varied with the different intensity light stimulation in hen, the immunoreactive intensity was the highest in 20Lux and 30Lux group, secondary in 40Lux, the lowest in 10Lux group within SGC, ROT, SLu, Glv, Imc and Ipc. That was highest in 20Lux group, gradually weakened in 30Lux and 40Lux group within PVN and ARC. The immunoreactive intensity was increased with the intensity of light within OMc. It indicated that the responses in central nervous system to the light stimulation vary with their intensity.