Abstract: applying the principles and methods of population genetics, number of wild rape and its close relative species in Tibet and genetic diversity indexes are researched among 100 wild rape and its close relative species in Tibet. The results show that: 1) The center of genetic diversity of wild rape and its close relative species in Tibet are shaped in the horizontal distribution of 28~31°N×88~94°E and 29~32°N×96~99°E. Wild rape and its close relative species in Tibet distribute richer and more wildly in the 28~31°N×88~94°E than in the 29~32°N×96~99°E. 2) In the vertical distribution, they shape three different central regions of genetic diversity which are separately 3500~3600m、3800~3900m、4000~4200m. There is critical band between rich region of genetic diversity in the low altitude and rich region of genetic diversity in the high altitude. 3) The center and diffusion pattern of genetic diversity of wild rape and its close relative species in Tibet is put forward according to number of wild rape and its close relative species in Tibet and genetic diversity indexes.