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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (14): 59-64.

所属专题： 生物技术

• 生物技术科学 • 上一篇    下一篇

桃果实硬核后期中果皮与内果皮中蛋白质组表达的双向电泳分析

杨爱珍 王一鸣 花宝光 刘悦萍 王有年   

  • 收稿日期:2009-09-25 修回日期:2009-10-09 出版日期:2010-07-20 发布日期:2010-07-20
  • 基金资助:
    北京市自然科学基金重点项目

A two-dimensional electrophoresis protocol to study protein discrepancies in mesocarp and putamen during prunus persica fruit development

  • Received:2009-09-25 Revised:2009-10-09 Online:2010-07-20 Published:2010-07-20

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摘要： 本研究以“大久保”桃（Prunus persica L.cv.Okubao）硬核后期（盛花后56 d）的果实为试材，固相pH梯度（IPG）双向电泳系统分离蛋白质，所得图谱在中果皮与内果皮中检测到蛋白质点的数量分别为1273±101和1292±115个，其中30个为内果皮相对于中果皮2倍（p<0.05）上调的点，35个为2倍（p<0.05）下调的点。有10个蛋白点仅在中果皮表达，18个蛋白点只在内果皮表达。

Abstract: Peach (Prunus persica L.cv.Okubao) fruits around 56 days after anthesis were adopted in this experiment. The total protein was extracted and isolated by immobilized pH gradient（IPG）based on 2-DE routine. 1273±101 and 1292±115 protein spots were detected respectively from 2-DE patterns of mesocarp and putamen. Many spots of aggregate matched protein spots were present in both patterns of mesocarp and putamen, and 30 spots showed 2-fold（t-test, p<0.05）up-regulated on quantitative appearing an increase in spot intensity and maximum abundance in putamen than mesocarp , in addition 35 protein spots expressed 2-fold（t-test, p<0.05）down-regulated. 10 spots were just observed in mesocarp’s pattern, whereas 18 spots was only found in putamen.