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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (3): 42-45.

所属专题： 生物技术； 畜牧兽医

• 生物技术科学 • 上一篇    下一篇

基于绵羊皮肤组织ESTs开发新型微卫星标记

王遵宝,努尔力·阿不力孜,赵宗胜,曾献存,吴洪宾   

  • 收稿日期:2009-10-13 修回日期:2009-10-22 出版日期:2010-02-05 发布日期:2010-02-05
  • 基金资助:

    绵羊非繁殖季节产羔性状的QTL定位及相关基因的分离

Identification of microsatellites from skin of sheep

  • Received:2009-10-13 Revised:2009-10-22 Online:2010-02-05 Published:2010-02-05

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643

摘要：

利用生物信息学方法，在从国际公共生物数据库检索获得绵羊皮肤组织相关的3295条EST序列中筛选微卫星标记。结果表明,从绵羊皮肤组织相关的EST序列中搜索到微卫星209个,含有微卫星的序列196 个,占整个EST 序列数据库的6.3% , 其中双碱基重复150个,三碱基重复19个,四碱基重复12个,五碱基重复17个,六碱基重复11个。在这些微卫星序列中,AC/TG重复在双碱基类型中最丰富,占双碱基微卫星序列总数的71.8%。根据筛选到的微卫星序列设计并合成引物22对 ,其中18 对引物有扩增产物,且条带清晰, 6对引物在中国美利奴羊群内多态性丰富,平均PIC达0.7001，为后期与毛品质进行相关性分析，寻找毛用性状新型分子标记奠定了基础。

关键词: 政治参与, 政治参与, 村委选举, 有序Probit模型, 南京

Abstract:

3,295 ESTs from NCBI were mined to develop EST-SSR markers.By using SSRHunter soft tools, a total of 209 SSRs were identified from 196 ESTs. The frequency of EST-SSRs was 6.3%. The dinucleotide repeat motif was the most abundant SSR, accounting for 71.8%, followed by 9.1%, 8.1%, 5.7% and 5.3%, respectively,for tri-, hexa-, penta- and tetra-nucleotide repeats. Among the di- nucleotide repeats, AC/TG (31%)were the most abundant type. 22 primer pairs were designed f rom microsatellite,18 pairs could show clear PCR products by electrophoresis,and 6 pairs show highly polymorphism in Chinese Merino sheep. The mean PIC value was 0.7001, ranging from 0.5499 to 0.7607.These EST-SSR markers can be used for further study such as in genetic mapping, identification of quantitative trait loci (QTLs),and comparative genomics studies of sheep.