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中国农学通报 ›› 2010, Vol. 26 ›› Issue (3): 46-51.

所属专题: 食用菌 食用菌

• 生物技术科学 • 上一篇    下一篇

有机酸和氨基酸对双孢蘑菇菌丝生长的影响

刘爱民,陈金风,张金金,方启,王敏,查琴   

  • 收稿日期:2009-09-09 修回日期:2009-09-21 出版日期:2010-02-05 发布日期:2010-02-05

Effect of Organic Acid and Amino Acid on the Mycelial Growth of Agaricus Bisporus

  • Received:2009-09-09 Revised:2009-09-21 Online:2010-02-05 Published:2010-02-05

摘要:

本实验利用固体平板和液体摇瓶培养的方法,研究了柠檬酸、酒石酸、琥珀酸三种有机酸和丙氨酸、酪氨酸、精氨酸三种氨基酸在不同浓度下对双孢蘑菇菌丝生长的影响。通过测定各处理组的菌丝菌落直径、胞外蛋白含量、菌丝干重、胞内多糖、过氧化物酶活性和还原糖含量等生长参数,结果表明,外源加入0.04%的柠檬酸、琥珀酸、酒石酸和精氨酸能够促进双孢蘑菇的生长,其中琥珀酸组的菌丝干重最高,比对照组增加了131%;精氨酸组的胞外蛋白含量最高,比对照组增加了151%;柠檬酸组的胞内多糖含量和过氧化物酶活性的值最高,分别比对照增加了21%和146%。但过高浓度的有机酸和氨基酸,则抑制双孢蘑菇菌丝的生长。

关键词: 射流器, 射流器, 数值模拟, 臭氧, 喉管, 优化设计

Abstract:

In the article, the effects of three kinds of organic acid from citric acid, tartaric acid and succinic acid and three kinds of amino acid from alanine, tyrosine and arginine in different concentration on the growth of Agaricus bisporus were studied by the solid and liquid culture methods. The growth parameters of the clony diameter, extracellular protein content, mycelium dry weight, intracellular polysaccharide content, peroxidase activity and reducing sugar content were analyzed in treat groups. The results showed that the growth of Agaricus bisporus could be promoted by adding 0.04% citric acid, succinic acid, tartaric acid and arginine. Compared to the control in the medium, the mycelium dry weight was highest by adding succinic acid, it increased 131%; the extracellular protein content was highest by adding arginine, it increased 151%; intracellular polysaccharide content and peroxidase activity of Agaricus bisporus were the highest value by adding citric acid, they increased 21% and 146% respectively. But higher concentration organic acid and amino acid would inhibit the growth of Agaricus bisporus.