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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (6): 260-264.

所属专题： 生物技术； 水稻

• 资源 环境 生态 土壤 气象 • 上一篇    下一篇

开放式臭氧浓度升高对水稻叶片呼吸作用相关酶的影响

梁晶1,2,曾青1,朱建国1,谢祖彬1,刘钢1,朱春梧1,2,曹际铃1,2,唐昊冶1   

  • 收稿日期:2009-11-02 修回日期:2009-12-02 出版日期:2010-03-20 发布日期:2010-03-20
  • 基金资助:

    日本环境厅全球环境研究基金项目（中日合作）;国家自然科学基金项目;中国科学院南京土壤研究所知识创新工程领域前沿项目;国家科技部国际科技合作计划项目;中国科学院科研装备研制项目;中国科学院国际合作重点项目

Effects of O3-FACE(ozone-free air control enrichment) on respiration enzymes of rice leaf

  • Received:2009-11-02 Revised:2009-12-02 Online:2010-03-20 Published:2010-03-20

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692

摘要：

本文在开放式臭氧浓度升高（ Ozone-free air controlled enrichment）条件下，以武运粳21（粳稻）和两优培九（杂交稻）两个耐性不同的品种为材料，研究了大气臭氧浓度升高对水稻叶片乙醇酸氧化酶（GO）、多酚氧化酶（PPO）和抗坏血酸氧化酶（AAO）三种呼吸作用相关酶的影响。结果表明：臭氧处理67天，促进了GO、PPO和AAO的活性，武运粳21 升高的幅度大于两优培九；在处理79天，臭氧胁迫抑制了水稻叶片GO、PPO和AAO的活性，品种间比较，武运粳21三种酶降低的幅度小于两优培九。试验结果说明，武运粳21在通过提高三种酶的活力而进行较强的呼吸作用，起到耗散过剩光能并保护光合器官的作用，从而缓解臭氧对光合器官的损害缓解臭氧对水稻叶片造成的伤害，因此武运粳21比两优培九对臭氧耐受性强。

关键词: OnyxFLOWER, OnyxFLOWER, 麝香百合, 建模

Abstract:

In this paper, two rice varieties(Wuyunjing21 and Liangyoupeijiu) were used to detect polyphenol oxidase (PPO), ascorbic acid oxidase (AAO), glycolic acid oxidase (GO) activities of leaves for heading date of rice (Oryza sativa I )under the ambient atmosphere O3 concentration (Ambient) and O3 free air concentration enrichment (O3-FACE) conditions. Results showed that: elevated O3 concentration enhance the activities of PPO, AAO and GO after 67 days O3 treatment，Wuyunjing 21 increased these enzymes activity much than those of Liangyoupeijiu; however, after 79 day O3 treatment, elevated O3 concentration decreased GO, PPO and AAO activity, especially these enzymes activity reduction of Liangyoupeijiu were much than those of Wuyunjing 21.The results indicated that, Wuyunjing 21 have strong respiration by enhancing three respiration enzymes activity to dissipate of excess light energy and protect the photosynthetic apparatus for alleviating the damage of ozone on the photosynthetic apparatus of rice leaves. So we concluded that Liangyoupeijiu was more susceptible to ozone than Wuyunjing 21.