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中国农学通报 ›› 2010, Vol. 26 ›› Issue (7): 261-266.

所属专题: 玉米

• 资源 环境 生态 土壤 气象 • 上一篇    下一篇

高效玉米秸秆降解菌复合系的构建

郭夏丽1,程小平2,杨小丽1,王岩1   

  • 收稿日期:2009-12-02 修回日期:2009-12-17 出版日期:2010-04-05 发布日期:2010-04-05

Construction of a composite consortia with high capacity of lingocellulose degradation

  • Received:2009-12-02 Revised:2009-12-17 Online:2010-04-05 Published:2010-04-05

摘要:

以秸秆降解率为指标,通过单菌种组配,得到由纤维素降解菌Y11-4和X2、木质素降解菌L3、酵母菌J6组成的秸秆降解菌组合:Y11-4+X2+L3+J6。以秸秆为唯一碳源进行限制性继代培养,依据pH恒定,得到5种分别来自腐烂秸秆、森林土、菜园土、竹林土及麦田土的驯化系。通过菌系组配,得到了一个来源于森林土、竹林土和腐烂秸秆的驯化系组合,其秸秆降解率为38.3%。进一步将驯化系与组合菌复配并限制性继代培养,以构建降解菌复合系,得到的秸秆降解复合菌剂在14天内秸秆降解率达到45.7%。

Abstract:

A mixture consisting of cellulose-decomposing fungi Y11-4 and X2, lignin-degrading fungus L3 and yeast J6 was obtained by strain combination according to the stalk-degrading efficiency. With maize stalk as sole carbon source, the five kinds of domesticated strains from forest soil, cornfield soil, bamboo soil, rotten cornstalk and garden soil were found by the techniques of limited cultivation and using pH as a criterion of stability. And a complex of domesticated strains from forest soil, bamboo soil and rotten corn stalk was prepared based on the straw-degrading efficiency. The straw-degrading efficiency of the complex was up to 38.3%. A efficient straw-degrading composite microbial system was constructed by mixing domesticated strains from forest soil, bamboo soil and rotten corn stalk with Y11-4+X2+L3+J6 and continuous limited subculture. And its straw degradation efficiency was up to 45.7% in 14 days