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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (10): 38-41.

所属专题： 生物技术； 水稻

• 生物技术科学 • 上一篇    下一篇

海南普通野生稻NBS类抗病基因同源序列的分离与分析

徐靖,云勇,唐清杰,王效宁   

  • 收稿日期:2009-12-07 修回日期:2009-12-18 出版日期:2010-05-20 发布日期:2010-05-20
  • 基金资助:

    国家自然科学基金项目;海南省自然科学基金项目;国家科技支撑计划课题

Isolation and Sequence Analysis of NBS-type Resistance gene Analogues from Hainan Common Wild Rice (Oryza rufipogon Griff)

Xu Jing, Yun Yong, Tang Qingjie, Wang Xiaoning   

  • Received:2009-12-07 Revised:2009-12-18 Online:2010-05-20 Published:2010-05-20

PDF (PC)

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摘要：

利用已知植物抗病基因NBS(Nucleotide binding site)序列中的保守区域设计简并引物，以海南普通野生稻基因组DNA为模板进行PCR扩增，通过T/A克隆、测序和序列分析, 共得到4条具有连续ORF的抗病基因类似物(Resistance gene analogues, RGAs)序列，它们之间核苷酸序列间的相似性系数在53.14%-99.81%之间，而相应推测的氨基酸序列间的相似性系数在39.08%-99.42%之间。氨基酸序列进行结构分析表明，它们包括“P-loop”、“Kinase-2a”、“Kinase-3a”和“GLPL”4个抗病基因所共有的保守模体，并且4条海南普通野生稻NBS-LRR类似物均属于nonTIR-NBS类抗病基因片段。

关键词: 无线数据传输, 无线数据传输, nRF2401, AT89C51

Abstract:

Degenerate primers based on conserved motif of the nucleotide binding site(NBS) region from the cloned plant disease resistance genes were used to isolate resistance gene analogues (RGAs) from genomic DNA of Hainan common wild rice (Oryza rufipogon Griff). After T/A cloning, sequencing and analyzing by alignment, 4 RGAs with uninterrupted open reading frames (ORFs) were obtained. Sequence identity among the 4 RGAs nucleotide sequences ranged from 53.14% to 99.81%, while the 4 RGAs deduced amino acid sequences showed identity ranged from 39.08% to 99.42%. The deduced amino acid sequences of RGAs contain the conserved domains such as P-loop, Kinase-2, Kinase-3a and GLPL, and the 4 RGAs all belonged to nonTIR-NBS type resistance gene segments.