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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (10): 30-37.

所属专题： 生物技术； 园艺

• 生物技术科学 • 上一篇    下一篇

中国野生葡萄抗寒基因的RAPD标记及其序列分析

张剑侠,熊燕,王跃进,朱自果   

  • 收稿日期:2009-12-11 修回日期:2010-01-06 出版日期:2010-05-20 发布日期:2010-05-20
  • 基金资助:

    国家科技支撑计划;国家科技支撑计划;国家现代农业产业体系

RAPD Markers Linked to the Hardiness Gene in Chinese Wild Vitis and Their Sequence analysis

Zhang Jianxia, Xiong Yan, Wang Yuejin, Zhu Ziguo   

  • Received:2009-12-11 Revised:2010-01-06 Online:2010-05-20 Published:2010-05-20

PDF (PC)

1276

摘要：

以抗寒性存在差异的83份葡萄种质及三个杂交组合玫瑰香(Vitis vinifera)×黑龙江实生(V.amurensis)、北醇 (V. vinifera ×V.amurensis) 自交、燕山-1 (V. yeshanensis) ×河岸-3 (V.riparia Beaumont)的F1代122株为试材，进行了中国野生葡萄抗寒基因RAPD标记的研究。通过对110个随机引物的筛选，获得了2个与中国野生山葡萄抗寒基因相连锁的RAPD标记S241-670和S238-800。通过克隆、测序，这两个RAPD标记的实际长度分别是717 bp和854 bp，因此重新命名为S241-717和S238-854，其序列的GenBank登录号分别为GQ338290和GQ338289。经序列分析，S241-717与29条欧洲葡萄全基因组鸟枪序列有77～97 %的同源性，S238-854与6条欧洲葡萄全基因组鸟枪序列有82～100 %的同源性；二者分别编码欧洲葡萄的一种假定蛋白。RAPD标记S241-717和S238-854可用于葡萄抗寒育种的早期分子标记辅助选择。

关键词: 南美斑潜蝇, 南美斑潜蝇, 发生, 防治

Abstract:

RAPD markers linked to the hardiness gene in Chinese wild Vitis were studied with 205 plants, including 83 grape germplasms of different hardiness, 122 F1 individuals from cross combinations Muscat Hamburg (Vitis vinifera)×Heilongjiang seedling (V.amurensis), Beichun (Muscat Hamburg×V.amurensis) selfing and Yanshan-1 (V. yeshanensis)×V. riparia Beaumont. Two RAPD markers, S241-670 and S238-800 linked to the hardiness gene derived from hardy V. amurensis were obtained by Screening of 110 random primers. Furthermore, cloning and sequencing of the RAPD markers were carried out. The length of the RAPD markers was actually 717 bp and 854 bp. Therefore, they were renamed as S241-717 and S238-854. Nucleotide sequence data of the RAPD markers had been submitted to the GenBank databases，and the accession number was GQ338290 and GQ338289 respectively. Through sequences comparison between the RAPD markers and GenBank, S241-717 and S238-854 show identity with 29 and 6 EST sequences from Vitis vinifera L. respectively. Also, they can code a hypothetical protein of grape respectively. RAPD markers S241-717 and S238-854 could be used as the evidence for marker- assisted selection in grape hardiness breeding.