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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (10): 25-29.

所属专题： 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

日粮VE水平对荷斯坦种公牛精液品质和血液抗氧化指标的影响

郎海芳1,2,林英庭1,王玲玲2,宋杰2,仲跻峰2,侯明海2   

  • 收稿日期:2010-02-05 修回日期:2010-02-25 出版日期:2010-05-20 发布日期:2010-05-20
  • 基金资助:

    山东省良种产业化项目《奶牛优秀种公牛培育与高效利用技术研究》;山东省良种产业化项目《奶牛优秀种公牛培育与高效利用技术研究》

Effect of Different Additional Levels of Vitamin E on Semen Quality and Serum Antioxidant Parameters in Holstein Bulls

Lang Haifang1, 2, Lin Yingting1, Wang Lingling2, Song Jie2, Zhong Jifeng2, Hou M   

  • Received:2010-02-05 Revised:2010-02-25 Online:2010-05-20 Published:2010-05-20

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952

摘要：

为探讨VE的不同添加水平对荷斯坦种公牛精液品质和血液抗氧化指标的影响，选取年龄和体重相近、采精正常的荷斯坦成年种公牛24头，单因子实验设计，采用随机区组法分为4组，每组6头牛，分别饲喂VE添加水平为30 mg/kg、150 mg/kg、300 mg/kg、600 mg/kg（按日粮干物质计）的试验日粮，试验期9个月。结果表明：①日粮中添加300mg/kgVE，种公牛的鲜精活力和精液密度显著高于30mg/kg添加组（P<0.05），但与150mg/kg和600mg/kg组间差异不显著（P>0.05）；种公牛的畸形率以添加300mg/kgVE组最低，极显著低于30mg/kg添加组（P<0.01），但与150mg/kg和600mg/kg添加组间差异不显著（P>0.05）；采精量和冻后活力各组间差异均无显著性差异（P>0.05）。②不同VE添加水平对荷斯坦种公牛血清中谷胱甘肽过氧化物酶（GSH-Px）、超氧化物歧化酶（SOD）、总抗氧化能力（T-AOC）、羟自由基（?OH）和丙二醛（MDA）的影响极显著（P<0.01）。综合分析表明：荷斯坦种公牛日粮中VE较为的适宜添加水平为300 mg/kg。

关键词: 大豆花叶病毒（SMV）, 大豆花叶病毒（SMV）, 症状, 株系

Abstract:

Twenty-four breeding Holstein bulls assigned randomly to four groups were conducted to study the effect of different additional levels of vitamin E on semen quality and plasma antioxidant parameters. This experiment involved 4 dietary treatments，consisting of four additional levels of vitamin E which was 30mg/kg, 150mg/kg, 300mg/kg , 600mg/kg within 9 months. The results showed that: Compared with the vitamin E level of 30 mg/kg, sperm concentration and motility in 300 mg/kg level were significantly increased (P<0.05), and sperm abnormalities rate decreased significantly (P<0.01). There were found no significant difference between any of two treatments in ejaculate and freeze-thawed motility (P>0.05). There were extremely significant differences in serum activity of GSH-Px, SOD and T-AOC (P<0.01), and serum contents of ?OH and MDA (P<0.01). It seemed that the optimal additional level of vitamin E of stud Holstein bull was 300 mg/kg.