Abstract:

Abstract: Rhizomes derived from hybrid seeds of Cymbidium sinense?Cymbidium lancifolium were employed to investigate the effects of basic medium, plant growth regulator, organic additives, activated carbon, carbon sources, cutting way and light intensity on shoot differentiation influencing of rhizome. The results showed that the basic medium had significant effect on shoot differentiation, the highest mean shoot differentiation rate were obtained by using 1/2MS as basic medium. The plant growth regulator had no significant effect on shoot induction rate, but significantly affected mean shoot differentiation rate, the highest mean shoot differentiation rate,130.00%, appeared when 2.0 mg/L 6-BA and 0.2 mg/L NAA adopted. The organic additives and 30 g/L maltose promoted the shoot differentiation of the rhizome, and supplement of 100 ml/L coconut water in medium significantly improved mean shoot differentiation rate. Activated carbon significantly inhibited shoot differentiation. Cutting way significantly influenced shoot differentiation, the best results were achieved by separation branches from the mother rhizome followed by without cutting, the results of cutting the rhizome into segments about 1 cm in length were the worst. The effect of light intensity on shoot differentiation of rhizome was significant, and the optimum light intensity was 500 lx．