欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (9): 70-73.

所属专题： 畜牧兽医

• 生物技术科学 • 上一篇    下一篇

野生平榛多糖对小鼠免疫活性的影响

邵红,孙睿,童灵影,邵海军   

  • 收稿日期:2009-11-30 修回日期:2009-12-21 出版日期:2010-05-05 发布日期:2010-05-05
  • 基金资助:

    龙葵碱对荷瘤小鼠癌周围淋巴管生成及钙粘蛋白基因表达的影响

Effect of wild-Hazel Polysaccharide on immunity activity of mice

Shao Hong, Sun Rui, Tong Lingying, Shao Haijun   

  • Received:2009-11-30 Revised:2009-12-21 Online:2010-05-05 Published:2010-05-05

PDF (PC)

977

摘要：

目前关于多糖的研究报道很多，但在榛子多糖方面的研究并不多见。对野生榛子多糖进行研究，能提高野生植物的科学综合利用，对进一步挖掘药用植物资源有着重要的现实意义。用苯酚-硫酸法测定从野生平榛的叶片、种仁及叶片的愈伤组织中提取的粗多糖含量。以上述各多糖液（12.5mg/mL）给小鼠灌胃，10d后对小鼠的免疫器官、腹腔巨噬细胞吞噬功能及血清溶血素含量进行测定，评价多糖的免疫生物活性。结果表明，榛叶多糖含量最高，高达（2.93±0.06）%，愈伤组织多糖次之为（2.26±0.01）%，而榛仁多糖含量最低仅为（0.40±0.08）%；榛仁多糖的免疫活性最强，愈伤组织多糖的免疫活性次之，榛叶多糖不具有免疫活性。

关键词: 农业资源, 农业资源, 调查, 分析

Abstract:

At present, the reports on polysaccharide are very frequent, however the reports on Hazel polysaccharide’s are seldom. Polysaccharide of wild hazelnut research, can improve the scientific utilization of wild plants, to further tap the resources of medicinal plants has important practical significance. The content of polysaccharide from wild-Hazel leaves(PHl)、wild-Hazelnut (PHn) and wild-Hazel leaves＇callus(PHc) were determined by phenol sulfonic spectrophotometry. Mice received respectively with PHl、PHn、Phc (12.5mg/mL) by gavage for ten days. After that, immune organ, the function of macrophages phagocytosis and the content of serolysin in mice were determined and their immunological activity were evaluated. The results showed, the average content of PHl was (2.93±0.06)%, which was the highest, Phc was the second, its content was(2.26±0.01)%, PHn content was the lowest, only(0.40±0.08)%. Among the three polysaccharide, PHn was the strongest on the immunological activity; the second was PHc; PHl had no remarkable effect on immunity.