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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (9): 74-77.

所属专题： 生物技术

• 生物技术科学 • 上一篇    下一篇

多基因表达载体KCTB转化宁夏枸杞的研究

朱永兴1,曹鹏1,许兴1,孟青青2,赵子丹2   

  • 收稿日期:2009-12-21 修回日期:2010-01-14 出版日期:2010-05-05 发布日期:2010-05-05
  • 基金资助:

    973项目;宁夏自然基金

The transformation of mutigene expressed vector for Lycium barbarum. L

Zhu Yongxing1, Cao Peng1, Xu Xing1, Meng Qingqing2, Zhao Zidan2   

  • Received:2009-12-21 Revised:2010-01-14 Online:2010-05-05 Published:2010-05-05

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摘要：

为研究多基因表达载体KCTB转化宁夏枸杞，本研究利用农杆菌介导技术，利用叶盘转化法，将构建有GUS、BAR、KC1、CAF14个功能基因的表达载体KCTB转化宁夏枸杞，并获得了23株抗性植株;GUS组织化学染色显示，在转基因植株的根、茎、叶中都有表达，PCR结果显示，分别检测到了BAR、KC1、CAF1基因，说明多基因表达载体KCTB已经完全整合到了宁夏枸杞的基因组中。

关键词: 耕地占补充平衡, 耕地占补充平衡, 形势, 特征, 中国

Abstract:

For researching transformation of Lycium barbarum. L ,the multigene expressed vector KCTB that constructed with four functional genes, GUS、BAR、KC1、CAF1 was introduced into Lycium barbarum. L mediated with agrobacterium tumefaciens. Analysis of transgenic plant by histochemical staining showed that the GUS was expressed predominately in leaves, and also lesser degree in roots. The result of PCR suggest that BAR、KC1、CAF1 was detected and also the KCTB was contained in genome of Lycium barbarum. L.