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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (9): 60-64.

• 生物技术科学 • 上一篇    下一篇

响应面分析法优化怀地黄多糖的微波提取工艺

原增艳,刘红记,刘新育,洪冲,蔡明川,王明道   

  • 收稿日期:2009-12-21 修回日期:2010-01-08 出版日期:2010-05-05 发布日期:2010-05-05
  • 基金资助:

    国家自然科学基金项目

Study on The Process of The Rehmannia glutinosa Libosch Polysaccharide by Microwave Assisted Extraction Using Response Surface Methodology

Yuan Zengyan, Liu Hongji, Liu Xinyu, Hong Chong, Cai Mingchuan, Wang Mingdao   

  • Received:2009-12-21 Revised:2010-01-08 Online:2010-05-05 Published:2010-05-05

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摘要：

为了优化怀地黄多糖的微波提取工艺，本文采用苯酚—硫酸比色法对怀地黄多糖含量进行测定，通过设定不同单因素研究了微波处理时间、微波功率和液料比对怀地黄多糖提取率的影响，以响应面分析法确定了微波提取怀地黄多糖的最佳工艺。结果显示：微波处理时间、微波功率和液料比对地黄多糖得率具有显著影响，其中在微波处理功率为720W，微波处理时间为210s， 料水比为1:6条件下，地黄多糖提取率可达到61.9%，并且该工艺有很好的重现性。

关键词: ETH, ETH, KT, 6-BA, 绿豆幼苗, 形态特征, 生化成分, 组织结构

Abstract:

Abstract: In order to obtain the optimum condition of the Rehmannia glutinosa Libosch polysaccharide microwave-assisted extraction techniques, the content of polysaccharide in Rehmannia glutinosa Libosch was measured by phenol-H2SO4 colorimetric method. This paper studied the effect of treatment time of microwave, power of microwave and rate of water to material through devised different factors. The optimum microwave extraction condition was determined by response surface analysis. The results showed the treatment time of microwave, power of microwave and the rate of material to material had a remarkable effect on obtain of polysaccharide in Rehmannia glutinosa Libosch. When the power of microwave was 720W, the treatment time of microwave was 210s, the rate of material to water was 6:1, the extraction rate of polysaccharide in Rehmannia glutinosa Libosch was 61.9%. This result also had a good reproducibility.