欢迎访问《中国农学通报》,

中国农学通报 ›› 2019, Vol. 35 ›› Issue (27): 65-71.doi: 10.11924/j.issn.1000-6850.casb18040097

所属专题: 园艺

• 林学 园艺 园林 • 上一篇    下一篇

生长素和赤霉素对刺果瓜幼苗生长发育的影响

司贺龙1, 刘玉卫1, 张金林2, 谷俊涛1, 刘 莉3, 李明慧3   

  1. 1.河北农业大学生命科学学院;2.河北农业大学植物保护学院农药系;3.河北省农业环境保护监测站
  • 收稿日期:2018-04-20 修回日期:2019-09-05 接受日期:2018-06-22 出版日期:2019-09-24 发布日期:2019-09-24
  • 通讯作者: 张金林
  • 基金资助:
    河北省科技支撑项目“河北省新外来入侵植物刺果瓜防控技术”(16226503D)。

Effects of Auxin and Gibberellin on Growth and Development of Sicyos angulatus Seedling

  • Received:2018-04-20 Revised:2019-09-05 Accepted:2018-06-22 Online:2019-09-24 Published:2019-09-24

摘要: [目的]刺果瓜(Sicyo angulatus L.)是外来入侵植物,对当地生态破坏严重,幼苗期是刺果瓜生命周期相对薄弱环节,研究其幼苗的生长机理,对遏制刺果瓜的繁殖和扩展有很大的帮助。[方法]本试验以刺果瓜幼苗为研究对象,设置四个不同浓度(3 μmol/L、30 μmol/ L、300 μmol/ L、3000 μmol/ L)的生长素(IAA)、生长素抑制剂三碘苯甲酸(TIBA)、赤霉素(GA3)、赤霉素抑制剂矮壮素(CCC)、IAA+GA和TIBA+CCC对刺果瓜长出第一片真叶的幼苗进行处理,通过测量6个时间点( 1天,2天,3天,5天,7天,9天)刺果瓜幼苗的株高、根长、鲜重、干重等生理特性,探究IAA和GA对刺果瓜幼苗的影响。[结果]结果表明:300 μmol/L的IAA处理植株株高与根长长势超过其他试验组与对照组,而3000 μmol/L的TIBA植株株高与根长得到明显的抑制。3000 μmol/L的GA3处理组株高、干重值、鲜重增长速度最快,为刺果瓜的最适浓度范围;3 μmol/L GA3处理,植株增长速度最慢;300 μmol/L CCC对刺果瓜的矮化作用最好,但干重、鲜重值增加;接近3000 μmol/L的CCC矮化作用较好,对干重、鲜重几乎无促进作用,为控制刺果瓜生长的最适浓度范围。浓度为3000 μmol/ L的TIBA+CCC处理对刺果瓜幼苗的影响极为显著,在喷洒激素6 h后就可以看到刺果瓜幼苗受到抑制出现萎蔫,刺果瓜有极强的抗逆性,所以在12 h后萎蔫状态消失,虽然仍受到抑制,但仍然缓慢生长着。9 d后,浓度为3000 μmol/l的TIBA+CCC处理刺果瓜幼苗仅长高5.53 cm,而对照组长高18.70 cm。浓度为3000 μmol/l IAA+GA处理的刺果瓜幼苗长高34.47 cm。浓度为3000 μmol/l的TIBA+CCC对刺果瓜的抑制作用极其显著。[结论]通过研究刺果瓜的生长机理,使用外源激素对刺果瓜的生长有较好的抑制作用,为抵抗外源入侵植物提供了新方法。

关键词: 白肋烟, 白肋烟, 晾房, 晾制, 风机, 温度, 相对湿度

Abstract: [Objective]Sicyos angulatus L. is an invasive plant that causes serious damage to the local ecology. The seedling stage is a relatively weak stage in the life cycle of Sicyo angulatus L. It is important to study the growth mechanism of its seedlings and it is of great help in curbing the propagation and expansion of Sicyo angulatus L. [method]In this study, Sicyo angulatus L. seedlings were used as study objects, and four different concentrations (3, 30, 300, and 300 μmol/L) of auxin (IAA) ,auxin inhibitor triiodobenzoic acid (4TLBA), gibberellin (GA3), gibberellin inhibitor CCC, IAA+GA, and TIBA+CCC were used to treat the seedlings of the first true leaves of Sicyos angulatus L. According to measure the plant height, root length, fresh weight, dry weight of Sicyos angulatus L. seedlings at six different time points (1 day, 2 days, 3 days, 5 days, 7 days, 9 days) to investigate the influence of IAA and GA3 on Sicyos angulatus L.seedlings. [result]The results showed that the plant height and root length of plants treated by 300 μmol/L IAA were higher than those of other experimental groups and the control group, while the plant height and root length of plants treated by 3000 μmol/L TIBA were significantly inhibited. The highest growth rate of plant height, dry weight, and fresh weight was observed in the 3000 μmol/L GA3 treatment group, which was the optimal concentration range of Sicyos angulatus L. The growth rate of plants was the slowest at the treatment of 3 μmol/L GA3; 300 μmol/L CCCC was the best for dwarfing effect on Sicyos angulatus L. but the dry weight and fresh weight were increased. The dwarfing effect of CCC near 3000 μmol/L was better ang it had almost no effect on the dry weight and fresh weight. So it was the most suitable concentration range for controlling the growth of Sicyos angulatus L. The treatment of 3000 μmol / L TIBA+CCC had a significant effect on Sicyos angulatus L. seedlings, and the wilting of seedlings were appeared after being sprayed for 6 h. The Sicyos angulatus L. had strong resistance, so the wilting was disappeared after 12 h. Although the growth of seedings were suppressed, but they were still slowly growing. After 9 d, the seedlings of Sicyos angulatus L. treated with 3000 μmol / L TIBA+CCC only grew by 5.53 cm. and the control group 18.70 cm. The seedling length of Sicyos angulatus L treated with 3000 μmol/L IAA+GA was 34.47 cm. The inhibitory effect of 3000 μmol / L TIBA+CCC on Sicyos angulatus L. was extremely significant. [Conclusion] By studying the growth mechanism of locust fruit, the use of exogenous hormones had a good inhibitory effect on the growth of fruit squash, which provided a new method for resisting invasive plants.

中图分类号: