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中国农学通报

中国农学通报 ›› 2020, Vol. 36 ›› Issue (35): 48-54.doi: 10.11924/j.issn.1000-6850.casb20191200991

所属专题： 资源与环境； 园艺

• 林学·园艺·园林 • 上一篇    下一篇

云南野生大理茶种质资源生化成分多样性分析

杨盛美(), 蒋会兵, 段志芬, 李友勇, 刘悦, 尚卫琼, 刘本英(), 唐一春()   

  1. 云南省农业科学院茶叶研究所/云南省茶学重点实验室,云南勐海 666200
  • 收稿日期:2019-12-24 修回日期:2020-02-06 出版日期:2020-12-15 发布日期:2020-12-18
  • 通讯作者: 刘本英,唐一春
  • 作者简介:杨盛美,女,1978年出生,云南昭通人,副研究员,研究方向：茶树种质资源与遗传育种研究。通信地址：666200 云南省西双版纳州勐海县勐海镇景囡街2号 云南省农业科学院茶叶研究所,Tel：0691-5170192,E-mail： yangshengmei102@qq.com。
  • 基金资助:
    国家自然科学基金项目“野生茶树雄性不育株细胞学及生理生化特性研究”(31760224);云南省科技厅重大科技专项计划“云南特色茶资源发掘利用及新品种繁育推广”(2018ZG009);云南省勐海县科技计划项目“勐海县野生茶树嫁接试验及加工适制性研究”(20180601)

Biochemical Compositions of Wild Tea Germplasm Resources (Camellia taliensis) in Yunnan: Diversity Analysis

Yang Shengmei(), Jiang Huibing, Duan Zhifen, Li Youyong, Liu Yue, Shang Weiqiong, Liu Benying(), Tang Yichun()   

  1. Tea Research Institute, Yunnan Academy of Agricultural Sciences/Yunnan Provincial Key Laboratory for Tea Science, Menghai Yunnan 666200
  • Received:2019-12-24 Revised:2020-02-06 Online:2020-12-15 Published:2020-12-18
  • Contact: Liu Benying,Tang Yichun

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摘要：

旨在分析云南野生大理茶种质资源生化成分多样性。本研究以21份云南野生大理茶种质资源为实验材料,对茶多酚、氨基酸、水浸出物、咖啡碱及儿茶素组成等生化成分含量进行测定,鉴定评价其生化成分多样性。结果表明,茶多酚含量为18.80%~25.20%、氨基酸总量为2.00%~3.90%、咖啡碱含量为1.10%~4.10%、水浸出物含量为45.30%~52.50%、儿茶素总量为4.37%~14.17%、变异系数范围为3.33%~157.89%,平均遗传多样性指数(H')为1.77。基于生化成分含量的聚类分析将21个野生大理茶分为2个类群,其中第1类群的C、EC、非酯型儿茶素及儿茶素总量含量较低;第2类群的EGCG、ECG、酯型儿茶素和儿茶素总量含量较高。云南野生大理茶种质资源生化成分含量的平均多样性指数较低,从中初步筛选出高茶多酚资源1份、低咖啡碱资源2份及高氨基酸资源1份,这些优异资源可以直接利用或作为茶树育种的优良亲本材料加以利用。

关键词: 云南, 大理茶, 生化成分, 多样性

Abstract:

This study aims to analyze the biochemical composition diversity of wild tea germplasm resources (Camellia taliensis) in Yunnan. The contents of tea polyphenols, amino acids, water extracts, caffeine and catechin from 21 wild tea germplasm resources (Camellia taliensis) in Yunnan were determined, and their biochemical diversity was also evaluated. The results showed that the tea polyphenols content ranged from 18.80% to 25.20%, the amino acids content ranged from 2.00% to 3.90%, the caffeine content ranged from 1.10% to 4.10%, the water extracts content ranged from 45.30% to 52.50%, the total catechin content ranged from 4.37% to 14.17%, the variation coefficient was 3.33%-157.89%, and the average genetic diversity index was 1.77. The cluster analysis of biochemical composition contents divided these 21 resources into 2 groups: the contents of C, EC, non-ester catechin and total catechins in group 1 were relatively low, while the contents of EGCG, ECG, ester catechin and total catechins in group 2 were relatively high. One germplasm with high polyphenols, two germplasms with low caffeine and one germplasm with high amino acids were preliminarily selected from the 21 wild tea germplasm resources, and these excellent resources could be utilized directly or as excellent parent materials for tea breeding.

Key words: Yunnan, Camellia taliensis, biochemical compositions, diversity

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