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中国农学通报 ›› 2021, Vol. 37 ›› Issue (11): 9-14.doi: 10.11924/j.issn.1000-6850.casb2020-0532

所属专题: 生物技术

• 生物科学 • 上一篇    下一篇

不同浓度细胞分裂素对甜菜种子引发的影响

刘小越1(), 王荣华2, 王维成2, 刘镎1, 刘晓晗1, 刘朝阳1, 刘大丽1, 吴则东1, 王茂芊1()   

  1. 1黑龙江大学现代农业与生态环境学院,哈尔滨 150080
    2新疆石河子农业科学研究院甜菜所,新疆石河子 832000
  • 收稿日期:2020-10-09 修回日期:2020-12-16 出版日期:2021-04-15 发布日期:2021-04-13
  • 通讯作者: 王茂芊
  • 作者简介:刘小越,女,1996年出生,安徽蚌埠人,在读硕士,研究方向:种子引发和甜菜分子遗传育种。通信地址:150080 黑龙江省南岗区学府路74号 黑龙江大学现代农业与生态环境学院,Tel:0451-86604650,E-mail: 970766396@qq.com
  • 基金资助:
    国家糖料产业体系子项目“甜菜种子引发与检测技术研究”(CARS-17011306);国家糖料产业体系子项目“甜菜抗根病及叶病品种鉴定与种质资源筛选”(CARS-17011004);国家青年科学基金项目“能源甜菜应答重金属Cd逆境胁迫分子机制的转录组学研究及差异表达基因功能鉴定”(31601229);国家糖料产业技术体系建设项目“甜菜高品质品种改良”(CARS-170111);黑龙江省自然科学基金项目“BvHIPP24基因在能源甜菜重金属镉污染生物修复中的分子机制研究”(LH2019C057)

Cytokinin Concentrations: Effect on the Germination of Sugarbeet Seeds

Liu Xiaoyue1(), Wang Ronghua2, Wang Weicheng2, Liu Na1, Liu Xiaohan1, Liu Zhaoyang1, Liu Dali1, Wu Zedong1, Wang Maoqian1()   

  1. 1College of Advanced Agriculture and Ecological Environment of Heilongjiang University, Harbin 150080
    2Research Institute of Sugarbeet, Xinjiang Shihezi Academy of Agricultural Sciences, Shihezi Xinjiang 832000
  • Received:2020-10-09 Revised:2020-12-16 Online:2021-04-15 Published:2021-04-13
  • Contact: Wang Maoqian

摘要:

为了测定不同浓度细胞分裂素6-BA的引发剂对甜菜种子引发的效果,为甜菜种子包衣剂物料筛选提供依据,本研究以TD802甜菜种子为试验材料,设置3种浓度和3个时间梯度的9个处理,通过对甜菜种子浸泡回干发芽培养,测定它们的发芽势、发芽率、发芽指数以及活力指数。结果显示,0.1%6-BA处理甜菜种子在8、12、24 h时的发芽势、发芽率、发芽指数和活力指数均较高于对照种子;0.5%6-BA不同时间处理甜菜种子,处理时间8 h和12 h的发芽势、发芽率、发芽指数和活力指数都高于对照;1%6-BA只有处理甜菜种子12 h时的发芽率、发芽指数和活力指数高于对照。不同浓度细胞分裂素6-BA对甜菜种子的萌发随着时间不同,效果也各有不同。处理时间过长或过短都会影响引发效果。

关键词: 甜菜, 细胞分裂素, 种子, 6-BA, 种子引发, 种子包衣剂

Abstract:

To determine the priming effect of different concentrations of cytokinin 6-BA on sugarbeet seeds, and to provide a basis for screening sugarbeet seed coating materials, in this study, TD802 sugar beet seeds were used as experimental materials, and 9 treatments with 3 concentrations and 3 time gradients were set up. The germination energy, germination rate, germination index and vigor index of beet seeds were determined by soaking and drying. The results showed that the germination energy, germination rate, germination index and vigor index of sugarbeet seeds treated with 0.1% 6-BA at 8, 12 and 24 h were higher than those of the control. The germination energy, germination rate, germination index and vigor index of sugarbeet seeds treated with 0.5% 6-BA for 8 h and 12 h were higher than those of the control. The germination rate, germination index and vigor index of sugarbeet seeds treated with 1% 6-BA for 12 h were higher than those of the control. Therefore, different concentrations of cytokinin 6-BA have different effects on the germination of sugarbeet seeds with different time, too long or too short treatment time could affect the triggering effect.

Key words: sugarbeet, cytokinin, seed, 6-BA, seed priming, seed coating agent

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