欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (11): 258-260.

所属专题： 园艺

• 林学 园艺 园林 • 上一篇    下一篇

霍山石斛试管丛生芽增殖影响因素的探讨

翟月婷,傅玉兰,姜亮   

  • 收稿日期:2010-01-08 修回日期:2010-02-27 出版日期:2010-06-05 发布日期:2010-06-05

Research of Influencing Factors in Multiple Shoot clumps Proliferation of Dendrobium huoshanense

Zhai Yueting, Fu Yulan, Jiang Liang   

  • Received:2010-01-08 Revised:2010-02-27 Online:2010-06-05 Published:2010-06-05

PDF (PC)

1084

摘要：

为了更好地保护霍山石斛野生资源，变野生为家种。本试验以霍山石斛试管丛生芽为试验材料，采用3因素3水平L9（33）正交设计方案，考察BA、NAA、KT不同激素及浓度对霍山石斛试管丛生芽继代增殖的影响。试验结果表明：植物激素BA、NAA、KT对霍山石斛试管丛生芽的增殖均有一定影响，其中NAA及KT的影响更为明显，并通过SAS软件统计分析确定三种植物激素的最佳浓度配比分别为：BA 1.5 mg/L，NAA 1.0 mg/L，KT 1.0 mg/L，即霍山石斛试管丛生芽继代增殖的最佳培养基配方为：MS+BA 1.5 mg/L+NAA 1.0 mg/L+KT 1.0 mg/L+食糖30.0 g/L+琼脂 8.0 g/L。

关键词: 品牌, 品牌, 品牌经营战略, 茶叶品牌, 六安瓜片

Abstract:

In order to protect the wild resources of Dendrobium huoshanense in better and change their home of wild species. In vitro shoots of Dendrobium huoshanense test material, using three factors, three level L9 (33) orthogonal design to study BA, NAA, KT of different hormones and concentration on the propagation of adventitious buds of Dendrobium huoshanense subculture proliferation. The results showed that: Plant hormones BA, NAA, KT right Dendrobium huoshanense proliferation of in vitro multiple shoot clumps have a certain impact, in which the impact of NAA and KT even more pronounced and, through the SAS statistical analysis software to determine the optimal concentration of three kinds of plant hormones ratio are as follows: BA use 1.5 mg / L, NAA use 1.0 mg / L, KT selected 1.0 mg / L, Namely, the best medium formula of Dendrobium huoshanense subculture in vitro multiple shoot clumps as follows: MS+BA 1.5 mg/L+NAA 1.0 mg/L+KT 1.0 mg/L+ Sugar 30.0 g/L+ Agar 8.0 g/L。