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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (11): 261-263.

所属专题： 玉米

• 植物保护 农药 • 上一篇    下一篇

玉米链格孢菌叶枯病病原菌的分子鉴定

周舒扬1,汪春蕾1,乔志新2,杨洪一1   

  • 收稿日期:2010-01-06 修回日期:2010-01-11 出版日期:2010-06-05 发布日期:2010-06-05

Identification of the Pathogen of Maize Alternaria leaf spot

Zhou Shuyang1, Wang Chunlei1, Qiao Zhixin2, Yang Hongyi1   

  • Received:2010-01-06 Revised:2010-01-11 Online:2010-06-05 Published:2010-06-05

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摘要：

对玉米链格孢菌叶斑病病原菌进行了分离、纯化，并按照柯赫氏法则证明其为玉米链格孢菌叶斑病病原菌。利用PCR技术对病原菌核糖体DNA内转录间隔区(internal transcribed spacer)进行扩增，并克隆、测序，测序结果显示其与GenBank中报道的细链格孢菌(Alternaria tenuis)同源性为99%，结合形态学特征，确定该病原菌为细链格孢菌。

关键词: 木薯, 木薯, 福建, 木薯产业

Abstract:

The pathogen of Maize Alternaria leaf spot was isolated and purified, and according to Koch's postulate, Alternaria leaf spot of maize was caused by the isolated fungi. The internal transcribed spacer (ITS) region of pathogen was amplified by polymerase chain reaction (PCR), and the specific fragment was cloned and sequenced. The result showed that the sequence of ITS for the pathogen shared 99% homology with that of Alternaria tenuis in GenBank. The pathogen was identified as Alternaria tenuis based on the morphological characteristics and sequence of ITS.