Abstract: Using conventional Japonica rice cultivar Nanjing 44 and hybrid Japonica rice combination Changyou 1, the effects of nitrogen (N) application rate ( 0 kg/hm2, 90 kg/hm2, 180 kg/hm2, 270 kg/hm2, 360 kg/hm2 ) on rice quality and starch RVA profile parameters of super Japonica rice were investigated under manual transplanting rice cultivation pattern. The results showed that rice milling quality, nutritional quality, cooking and taste quality, three peak starch RVA profile parameters (peak viscosity, hot viscosity, cool viscosity) of super Japonica rice measured in this study were significantly influenced by N application rate treatments, and the other starch RVA profile parameters differed between two super Japonica rice cultivars. Compared with Nanjing 44, rice appearance quality of Changyou 1 was more sensitive to N treatments, increasing N application rate would increase chalkiness rice rate and chalkiness degree of Changyou 1. Both Nanjing 44 and Changyou 1 showed the same trend that, with raising N application rate, brown rice rate, milled rice rate, head rice rate and rice protein content increased, while rice amylase content, taste value, peak viscosity, hot viscosity, cool viscosity of rice starch decreased. These results indicated that increasing N application rate would result in increment of milling quality and nutritional quality of rice, while rice taste quality and starch viscosity decreased.