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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (19): 280-284.

• 植物保护 农药 • 上一篇    下一篇

柑橘绿霉病菌拮抗放线菌MY-5的筛选与鉴定

李辉 耿鹏 郝卫宁 胡美英   

  • 收稿日期:2010-04-28 修回日期:2010-06-05 出版日期:2010-10-05 发布日期:2010-10-05
  • 基金资助:

    国家自然科学基金项目;广东省科技计划项目

Screening and identification of Actinomyces MY-5 antagonistic against Penicillium digitatum Sacc.

  • Received:2010-04-28 Revised:2010-06-05 Online:2010-10-05 Published:2010-10-05

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摘要：

从广东省各地区水果园和蔬菜地采集土壤23份，采用稀释平板法分离获得放线菌212株。以柑橘绿霉病菌为目标菌，采用抑菌带测定法，筛选获得9株具有拮抗活性的菌株，其中菌株MY-5拮抗活性最强，抑菌带宽达18.67mm，同时对其它13种果蔬采后病害也有较强的拮抗作用。通过对菌株MY-5形态观察、培养特征、生理生化反应和16SrDNA鉴定，该菌株为链霉菌属吸水类群（Streptomyces hygroscopicus）。

关键词: 巴西橡胶树, 巴西橡胶树, K+通道基因, HbKCO1, 高效表达

Abstract:

Two hundred and twenty-one actinomycetes strains were obtained from twenty-three soil samples collected from fruit orchards and vegetable fields in different parts of Guangdong Province by routine methods. 9 strains showed antagonistic activities against Penicillium digitatum Sacc. Among them，strain MY-5 exhibited the strongest antibiosis capacity with an inhibitory zone of 18.67 mm. Strain MY-5 was identified as Streptomyces hygroscopicus according to its 16SrDNA sequence analysis，and the morphological，physiological and biochemical characteristics.