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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (23): 93-96.

所属专题： 园艺

• 生物技术科学 • 上一篇    下一篇

不结球白菜亮白叶再生体系的优化

吕艳艳 王桂萍 沈振国 夏妍   

  • 收稿日期:2010-04-30 修回日期:2010-06-04 出版日期:2010-12-05 发布日期:2010-12-05
  • 基金资助:

    高等学校博士学科点专项科研基金项目

Optimization of Regeneration System in vitro of non-heading Chinese cabbage

  • Received:2010-04-30 Revised:2010-06-04 Online:2010-12-05 Published:2010-12-05

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摘要：

摘 要：本文以不结球白菜品种亮白叶为实验材料，研究不同外植体类型和不同生长调节剂配比等因素对其不定芽分化频率的影响。结果表明，对不结球白菜品种中再生比较困难的品种亮白叶来说，以下胚轴、子叶及子叶柄为外植体的诱导频率存在明显差异。以生长5-6天无菌苗的子叶柄为外植体，在培养基为MS+6-BA（5 mg/L）+NAA（0.1 mg/L）+ AgNO3（4mg/L）的培养条件下，亮白叶分化不定芽的再生频率最高，可达59.5%；这为将来该不结球白菜转化体系的建立奠定了坚实基础。

关键词: 野生狗牙根, 野生狗牙根, 均匀设计, SRAP-PCR, 体系优化

Abstract:

Abstract：we discussed the effect of explant types and the different combinations of 6-BA and NAA concentrations on the regeneration of the non-heading Chinese cabbage (Brassica campestris ssp. Chinensis L.) called Liangbaiye. The results showed that there was marked difference in the regeneration frequency between petiole with cotyledon, cotyledon and hypocotyl. The regeneration frequency of petiole with cotyledon was the highest among three explant types in the medium of MS + 6-BA (5.0 mg/L) + NAA (0.1mg/L) + AgNO3 (4.0 mg/L), and the differentiation frequency could reach as high as 59.5 %. That layed the foundation for establishment of genetic transformation system of Liangbaiye. Keywords: Chinese cabbage; adventitious buds; regeneration