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中国农学通报 ›› 2010, Vol. 26 ›› Issue (23): 168-172.

所属专题: 生物技术 小麦

• 农学 农业基础科学 • 上一篇    下一篇

赤霉菌粗毒素对小麦幼苗MDA含量和保护酶活性的影响

吴美金 王敏 张从宇   

  • 收稿日期:2010-05-25 修回日期:2010-06-28 出版日期:2010-12-05 发布日期:2010-12-05
  • 基金资助:

    安徽省教育厅自然科学重点项目

Effects of Fusarium graminearum Crude Toxin on MDA Contents and Protective Enzyme Activities in the Seedling of Wheat

  • Received:2010-05-25 Revised:2010-06-28 Online:2010-12-05 Published:2010-12-05

摘要:

为了探讨不同赤霉病抗性小麦品种对赤霉菌粗毒素胁迫的生理生化反应的差异及其与抗病性的关系,用赤霉菌粗毒素处理赤霉病抗性不同的小麦品种的幼苗,在0.5~4天内测定各品种的丙二醛(MDA)含量、超氧化物歧化酶(SOD)及过氧化物酶(POD)活性的增率。结果表明,处理后感病品种幼苗的MDA含量增率高于抗病品种和中抗品种。各品种幼苗的SOD活性先增加后下降,抗性强的品种SOD活性的增率大,活性下降慢;抗性较弱的品种SOD活性的增率小,活性下降快。各小麦品种幼苗叶片的POD活性增率在1~4天内均呈升高趋势,抗性强的品种POD活性的增率小,抗性弱的品种POD活性的增率大。对MDA含量增率、SOD及POD活性增率的第2天测定值和第2~4天测定均值作方差分析表明,三种不同抗性品种间差异显著。

关键词: 嗜热链球菌, 嗜热链球菌, 胞外多糖, 纯化条件

Abstract:

To clarify the difference of the physiological response of different wheat varieties to the stress of Fusarium graminearum crude toxin and it’s relationship with Fusarium head blight resistance, the seedlings of different wheat varieties were treated with Fusarium graminearum crude toxin. The incremental ratios of MDA contents, SOD and POD activities of seedlings were tested in 0.5~4 days after treatment. The experimental results showed that the incremental ratios of MDA contents in the seedlings of the highly resistant and moderately resistant varieties were lower than susceptible varieties. SOD activity of all varieties increased after treatment and then decreased in the second day after treatment, SOD activity incremental ratios were higher in highly resistant and moderately resistant cultivars than in susceptible varieties. POD activity increased in all kind of varieties in 1~4 days after treatment, but more quickly in susceptible cultivars than in highly resistant ones. There was remarkable difference among different resistant wheat varieties by variance analysis of the second day’s observations and the mean of 2~4 days’ observations of the incremental ratios of MDA content, SOD and POD activities.

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