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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (1): 42-44.

所属专题： 生物技术； 畜牧兽医； 小麦

• 农学 农业基础科学 • 上一篇    下一篇

欧山羊草高分子谷蛋白亚基向小麦的转入及其遗传规律

周建平   

  • 收稿日期:2010-06-21 修回日期:2010-07-26 出版日期:2011-01-07 发布日期:2011-01-07
  • 基金资助:

    国家科技支撑计划项目

Transferred HMW Glutenin Subunits from Aegilops biuncialis to Common wheat and Its Genetic Analysis

  • Received:2010-06-21 Revised:2010-07-26 Online:2011-01-07 Published:2011-01-07

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摘要：

欧山羊草具有两个容易用聚丙烯酰胺凝胶电泳与小麦区别的高分子谷蛋白亚基，分别命名为Glu-Aeb1和Glu-Aeb2。采用聚丙烯酰胺凝胶电泳（SDS-PAGE）方法对21份欧山羊草与小麦杂交后代BC2F6和15份BC2F5与小麦杂交F0种子的谷蛋白亚基组成进行了鉴定。实验结果表明，36份材料全部都有欧山羊草高分子谷蛋白亚基的转入。Glu-Aeb1在BC2F6和杂交组合F0中的传递频率分别为59.52%和20.94%。而Glu-Aeb2在BC2F6和杂交组合F0中的传递频率分别为40.48%和22.96%。

关键词: 陆羽, 陆羽, 《茶经》, 生态美

Abstract:

Two HMW glutenin subunits in Aegilops biuncialis, named with Glu-Aeb1 and Glu-Aeb2,can be easily identified from common wheat using polyacrylamide gel electrophoresis. 21 Aegilops biuncialis and common wheat hybrid progenies（BC2F6）along with 15 hybrids（F0）of BC2F5 and common wheat hybrid progenies were studied using SDS-PAGE. The results showed that all the materials are in possession of Aegilops biuncialis HMW glutenin subunits. The transfer frequency of Glu-Aeb1 in BC2F6 and F0 are 59.52% and 20.94%,respectively. While the transfer frequency of Glu-Aeb2 in BC2F6 and F0 are 40.48% and 22.96%, respectively．