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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (6): 107-111.

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

磷脂酶D对拟南芥抗冻性的影响

赵鹏 王道龙   

  • 收稿日期:2010-08-30 修回日期:2011-01-10 出版日期:2011-03-20 发布日期:2011-03-20
  • 基金资助:

    细胞膜磷脂水解酶在植物低温驯化过程中的作用与机理

Study on the Effect of Phospholipase D on the Freezing Tolerance of Arabidopsis thaliana

  • Received:2010-08-30 Revised:2011-01-10 Online:2011-03-20 Published:2011-03-20

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摘要：

为了探索磷脂酶D（PLD）在调控植物抗冻性中的作用，进一步揭示植物抗冻机理和磷脂低温信号转导机制的研究。笔者应用人工气候霜箱，对PLDγ1、PLDγ3基因分别被敲除的拟南芥突变体及野生型材料，进行低温驯化和冻害胁迫处理。试验发现，这2个基因的敲除型无论是经过还是未经过低温驯化冻害处理的离子渗漏率都与相同处理野生型拟南芥的离子渗漏率无显著差异。试验结果表明，PLDγ1和PLDγ3这2个基因既未参与组成型调控植物的抗冻性，也未参与低温信号转导过程。

关键词: C4途径, C4途径, PEPC, 抗旱生理, 转基因育种

Abstract:

The experiment was purposely to research the effect of phospholipase D on the freezing tolerance of Arabidopsis thaliana, reveal the mechanism of plants freezing tolerance and phospholipid signal transduction. Arabidopsis thaliana mutant PLDγ1 or PLDγ3 was deficient and wild-type was used as materials. The two mutants and wild-type were cold acclimated and freezing stressed using a climate chamber. There were no significant differences between the ion leakage of the mutants and wild-type whether cold acclimation freeze or non-acclimation freeze. The results showed that PLDγ1 and PLDγ3 neither mediate regulation of constitutive freezing tolerance nor low temperature signaling.