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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (7): 356-359.

所属专题： 园艺

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

动物源金黄色葡萄球菌的分离鉴定及其耐药性分析

邱梅   

  • 收稿日期:2010-10-21 修回日期:2010-12-03 出版日期:2011-04-05 发布日期:2011-04-05

Isolation and Identification of Staphylococcus aureus from Animals and Drug Resistance Analysis

  • Received:2010-10-21 Revised:2010-12-03 Online:2011-04-05 Published:2011-04-05

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摘要：

摘 要：为了了解动物源金黄色葡萄球菌的耐药情况，采集不同地区动物源临床样品，采用科玛嘉金黄色葡萄球菌显色培养基对样品中的金葡菌进行分离鉴定，微量肉汤稀释法对分离出的部分金葡菌进行耐药性分析。结果显示，青岛、济南和扬州等地犬源及山东平度鸡源总样品中金葡菌的分离率分别为8.2%、9.5%、5.8%、3.3%；犬源金葡菌对苯唑西林钠、头孢唑林钠、头孢曲松钠、氨苄西林钠、阿莫西林+克拉维酸钾、庆大霉素和阿米卡星的耐药率较低，而对磺胺甲噁唑钠、林可霉素、青霉素钾、硫氰酸红霉素、磷酸替米考星的耐药率均高于68.0%；鸡源金葡菌对多数药物产生高度耐药性，仅对庆大霉素和阿米卡星的耐药率较低，均为25%。表明，动物源金葡菌的分离率较低，鸡源金葡菌的分离率低于犬源临床样品；不同地区动物源金葡菌对临床常用药物产生了不同程度的耐药性，与犬源金葡菌的耐药情况相比，鸡源金葡菌的耐药性范围大、耐药程度严重。本研究为这些地区临床用药提供了一定的参考。

关键词: 冷冻精液, 冷冻精液

Abstract:

Abstract: To study the drug resistance of Staphylococcus aureus from animals, the S. aureus were isolated and identified from four clinical samples which were collected from the pet hospitals in Qingdao, Jinan, Yangzhou and a chicken farm in Pingdu with S. aureus color culture medium. The drug resistance of S. aureus analysis was carried out by mini-broth dilution susceptibility test. The results showed that the isolating rates of S. aureu from the pet dogs in Qingdao, Jinan, Yangzhou and the chicken in Pingdu were 8.2%, 9.5%, 5.8% and 3.3%, respectively. The drug resistance rates of S. aureus strains isolated from pet clinical samples to oxacillin sodium, cefazolin sodium, ceftriaxone sodium, ampicillin sodium, amoxicillin and clavulanate potassium, gentamycin and amikacin were low relatively. The resistance rates of S. aureus strains isolated from pet clinical samples to sulfamethoxazole sodium, lincomycin, benzylpenicillin sotassium, erythromycin thiocyanate, tilmicosin phosphate were more than sixty-eight percent. In the other hands, the resistance rates of S. aureus strains isolated from chicken to gentamycin, amikacin were 25.0%, 25.0%, respectively. It showed that the isolating rate of strains from chicken was lower than that from the dog pets, On the contrary, the drug resistance of S. aureus strains was higher.