欢迎访问《中国农学通报》,

中国农学通报 ›› 2019, Vol. 35 ›› Issue (18): 160-164.doi: 10.11924/j.issn.1000-6850.casb18030008

所属专题: 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    

我国牛冷冻精液质量性状的评估与分析

赵俊金1, 王振云2, 金穗华2   

  1. 1.全国畜牧总站;2.农业部牛冷冻精液质量检测中心南京
  • 收稿日期:2018-03-02 修回日期:2018-05-16 接受日期:2018-06-22 出版日期:2019-07-01 发布日期:2019-07-01
  • 通讯作者: 金穗华
  • 基金资助:
    云南省科技厅生物重大专项(奶业专项)项目-优秀种牛繁育及优质冻精推广(2014ZA007)。

Bovine Frozen Semen in China: Evaluation and Analysis of the Quality Traits

  • Received:2018-03-02 Revised:2018-05-16 Accepted:2018-06-22 Online:2019-07-01 Published:2019-07-01

摘要: 为进一步探索冷冻精液的质量提高与技术关键,本研究对近年来国内各牛冷冻精液生产企业1720头份精液样品进行了质量分析,涉及品种有奶牛、肉牛类(包括乳肉兼用、黄牛、牦牛等)和奶水牛等。结果:剂量值(0.20±0.008)mL、活力(40.9±2.3)%、每剂量前进运动精子数(1224.3±291.7)万个、畸形率(12.6±3.8)%、细菌菌落数(38.9±192.7)个。年度间比较,活力呈不规则的起伏变化;畸形率2016年均值显著低于前二个年度,分别下降0.8%和1.3%,3个年度间均差异显著(P<0.05),细菌菌落数2014年显著高于其他二个年度(P<0.05);品种间比较,奶牛活力分别高于奶水牛0.5%、肉、兼用牛0.2%(P<0.05),奶水牛与肉牛及兼用牛之间差异不显著(P>0.05),奶水牛畸形率分别高于奶牛1.9%、肉用牛0.9%,均差异显著(P<0.05),前进运动精子数,奶水牛最高,奶牛最低,品种间均差异显著(P<0.05);生产企业间比较,活力、前进运动精子数、畸形率均分别存在不同差异。通过数据分析,反映了目前国内牛冷冻精液的质量概况,为冷冻精液的技术研究提供依据。

关键词: 灌木, 灌木, 营养成分, 中性洗涤纤维

Abstract: Abstract: In order to further explore and improve the quality of frozen semen and the relative key technique, the quality of 1720 semen samples including dairy cattle, beef cattle (e.g. cattle, yellow cattle, yak, etc) and dairy buffalo, etc. from the domestic bovine frozen semen production enterprises had been analyzed in this research. It showed that the dose value was 0.20 ± 0.008 ml, the motility index was 40.9 ± 2.3%, the progressive motility sperm amount per dose was 1 224.3 ± 2.97 million, the abnormal sperm percentage was 12.6 ± 3.8% and the bacterial count was 38.9 ± 192.7. Motility index showed irregular interannual fluctuation changes, the abnormal sperm percentage in 2016 remarkable decreased by 0.8 and 1.3 respectively, compared with the previous two years. The difference during the three years was significant (P<0.05). The bacterial count in 2014 was significantly higher than that in the other two years (P<0.05). By comparison among breeds, the motility of dairy cattle increased by 0.5% than that of milk buffalo, by 0.2% than beef cattle and cattle (P<0.05). There was no significant difference between milk buffalo, beef cattle and cattle (P > 0.05). The abnormal sperm percentage of milk buffalo increased by 1.9% and 0.9% respectively than that of dairy cattle and beef cattle, and the difference is significant among the breeds (P<0.05). As for the progressive motility sperm amount per dose, milk buffalo was maximum, dairy cattle was minimum, and the difference among the breeds was significant (P<0.05). There were distinct differences in the quality index including motility, the progressive motility sperm amount per dose, the abnormal sperm percentage of the samples from different comprises. The above data analysis reflects the general situation of the quality of bovine frozen semen at present in China, and provides the basis for the technical research of frozen semen. Key words: Bulls; frozen semen; quality; evaluation; analysis