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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (30): 226-230.doi: 10.11924/j.issn.1000-6850.2012-0249

所属专题： 油料作物

• 植物保护 农药 • 上一篇    下一篇

芝麻成株期茎点枯病原菌致病性研究

黎冬华 张艳欣 王林海 吕海霞 危文亮 齐小琼 丁霞 张秀荣   

  • 收稿日期:2012-02-01 修回日期:2012-04-23 出版日期:2012-10-25 发布日期:2012-10-25
  • 基金资助:

    农业部油料作物生物学重点开放实验室开放课题“我国芝麻主要病害病原菌的分化”;国家青年科学基金“芝麻茎点枯病抗性全基因组关联分析及有效分子标记研究”

Pathogenicity of Macrophomina phaseolina to mature plant tissues of sesame (Sesamum indicum L.)

  • Received:2012-02-01 Revised:2012-04-23 Online:2012-10-25 Published:2012-10-25

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694

摘要：

本研究通过对感茎点枯病芝麻植株进行病原菌分离纯化及鉴定（包括表型鉴定、分子鉴定以及芽期致病力鉴定）获得了具有高致病力的病原菌株M. phaseolina，然后对获得的M. phaseolina进行芝麻成株期致病性研究，并对感病植株进行病原菌再分离鉴定从而确定获得的真菌为M. phaseolina。芝麻茎点枯病原菌M. phaseolina的致病性鉴定方法采用受侵染牙签接种法，在芝麻茎杆内，M. phaseolina会导致维管束组织形成一个由上往下逐渐坏死的棕黑色条纹，随后开始呈放射性收缩并坏死并逐渐扩大；所有病原菌接种的茎杆上都出现大量的菌核。对所有接种病原菌的植株感病茎杆进行M. phaseolina再分离纯化。茎点枯病原菌的分离鉴定、致病性鉴定以及病原菌再分离结果都符合科赫法则，为芝麻茎点枯病相关研究提供方法支持。

关键词: 延庆, 延庆

Abstract:

The pathogen causing sesame charcoal rot were collected, isolated, purified and identified (including phenotype analysis, molecular characterization and pathogenicity identification in the sprouting stage), and the Macrophomina phaseolina with strong pathogenicity was obtained in this study. Then the M. phaseolina pathogencity was evaluated in the inoculated tissues of mature plants with infecting toothpick pieces. In sesame stems, M. phaseolina caused brown-black necrotic streaks along vascular tissues, and then the streaks began to shrink radioactively and undergo local necrosis. Finally, M. phaseolina caused extensive necrosis. Sclerotium can also be found in the stems inoculated with pathogenic bacteria. All isolates of M. phaseolina were isolated from the inoculated stems and grown in pure culture again. These results fulfilled Koch’s postulates for M. phaseolina as a pathogen of mature sesame in China. These findings lay an important foundation for sesame resistance research.

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