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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (1): 13-16.doi: 10.11924/j.issn.1000-6850.2012-3055

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

银木的组织培养及植株再生技术研究

戴小英 宋晓琛 程强强 江香梅 肖复明   

  • 收稿日期:2012-09-07 修回日期:2012-10-25 出版日期:2013-01-05 发布日期:2013-01-05
  • 基金资助:
    江西省重大科技专项“樟科主要药用、香料、油用植物定向培育及产业化技术”(2010AZX00500)；江西省林业科学院青年基金项目“沉水樟等5种木本植物组培繁育技术研究”(2011521102)。

Tissue Culture and Plant Regeneration of Cinnamomum platyphyllum

  • Received:2012-09-07 Revised:2012-10-25 Online:2013-01-05 Published:2013-01-05

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摘要： 以银木2年生小苗的茎段为外植体，建立银木的组织培养和再生体系。研究不同种类、不同浓度生长调节剂、光照条件等因素对银木组培各阶段产生的影响。结果表明：适合嫩茎段腋芽初诱导与生长对培养基的选择不甚严格，在基本培养基MS中即可诱导；增殖培养基为MS+ BA 0.5 mg/L+ NAA 0~ 0.05 mg/L，增殖系数可达3.5以上；生根的最佳培养基为1/2MS+ NAA 0.1 mg/L + IBA 0.2 mg/L，生根率达98%以上。银木的高效快繁体系建立为银木的开发利用和工厂化育苗提供可靠技术依据。

关键词: 基因工程, 基因工程

Abstract: Cinnamomum platyphyllum tissue culture and regeneration system was established based on two young stem segments for explants. Research different kinds, concentrations of growth regulators and illumination conditions on affect of cultivation each stage. The results showed that the appropriate media for the axillary bud induction and germinate from young stem segments was MS. The optimal medium for multiplication was MS+ BA 0.5 mg/L + NAA 0-0.05 mg/L, and the regenerating coefficient was above 3.5. The medium for the rooting was 1/2MS+ NAA 0.1 mg/L + IBA 0.2 mg/L, and the rate reached above 98%. Establish the rapid propagation of Cinnamomum platyphyllum providing the reliable technical basis for utilization and factory production.