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中国农学通报 ›› 2014, Vol. 30 ›› Issue (9): 15-21.doi: 10.11924/j.issn.1000-6850.2013-1958

所属专题: 生物技术 农业工程 马铃薯

• 农学 农业基础科学 • 上一篇    下一篇

马铃薯Y病毒siRNA介导抗病基因工程研究进展

张威 白艳菊 范国权 高艳玲 申宇 张抒 郭炜 孟宪欣   

  • 收稿日期:2013-07-17 修回日期:2013-08-11 出版日期:2014-03-25 发布日期:2014-03-25
  • 基金资助:
    黑龙江省自然科学基金项目“马铃薯Y病毒N:O株系外壳蛋白基因siRNA介导病毒抗性研究”(C201234);黑龙江省农业科技创新工程重点资助项目“利用基因沉默siRNA策略实现抗马铃薯Y病毒研究”(2012ZD015);现代农业产业技术体系专项资金资助“国家马铃薯产业技术体系”(CARS-10-P14)。

Research Progress on Potato Virus Y siRNA-mediated of Disease Resistance Genetic Engineering

  • Received:2013-07-17 Revised:2013-08-11 Online:2014-03-25 Published:2014-03-25

摘要: 马铃薯病毒病是危害马铃薯生产的重要因素,尤其马铃薯Y病毒(Potato virus Y,PVY)严重影响马铃薯产量和品质,为防治带来很大困难。近年来,通过基因工程技术培育抗病毒转基因植株为抵抗PVY侵染提供有效途径。简述了目前PVY病毒siRNA介导抗病基因工程的主要方法策略,包括PVY不同功能基因介导的病毒抗性差异、影响PVY抗病毒基因工程效率的主要因素以及PVY不同株系联合抗病基因工程研究概况,分析了这些方法存在问题及发展趋势,旨在为更好地控制PVY危害提供借鉴。

关键词: 相关性, 相关性

Abstract: Potato viruses is an important factor to harm potato production, especially, potato virus Y (PVY) seriously affect the yield and quality of potato, and bring great difficulties for the prevention. In recent years, an effective way for resistance to PVY infection had been provided through genetic engineering technology to cultivate transgenic plants. The author outlines the main methods and strategies of the current PVY siRNA-mediated of disease resistance genetic engineering, including the differences of different PVY functional gene mediated virus resistance, the main factors affecting the efficiency of PVY antiviral gene engineering and the general of PVY different lines joint resistance genetic engineering, and analysis the problems and development trend of these methods, in order to provide reference for better control over the hazards of PVY.