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中国农学通报 ›› 2013, Vol. 29 ›› Issue (14): 26-30.doi: 10.11924/j.issn.1000-6850.2012-3346

所属专题: 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

福建黄兔对细菌内毒素的敏感性实验研究

俞春英 周建华 王训立   

  • 收稿日期:2012-10-10 修回日期:2012-10-26 出版日期:2013-05-15 发布日期:2013-05-15

Study on the Sensitivity of the Fujian Rabbits Response to Bacterial Endotoxin

  • Received:2012-10-10 Revised:2012-10-26 Online:2013-05-15 Published:2013-05-15

摘要: 为了解福建黄兔的体温情况以及对细菌内毒素的敏感性状况,探讨其是否适用于家兔的热原实验,按照《中国药典》2010年版中热原检测的标准,对福建黄兔进行体温预检筛查,合格家兔分组耳静脉注射浓度为5 EU/mL、10 EU/mL、20 EU/mL的细菌内毒素国家标准品,分析福建黄兔对内毒素的敏感性。结果表明:福建黄兔预检温筛选合格率为65.85%,体温主要分布于38.5~39.5℃。与国内报道的普通级新西兰兔筛选合格率相当,比普通级日本大耳白兔、普通级WHBE兔及SPF级新西兰兔稍低。福建黄兔注射不同浓度的细菌内毒素呈现不同的升温幅度,对细菌内毒素的敏感性与其浓度呈正相关,浓度越大,敏感性越高。通过本实验可知,福建黄兔体温较为恒定,对细菌内毒素也表现出一定的敏感性,若按实验用兔标准饲养,质量将更为稳定,可以探讨应用于热原试验,以拓宽福建黄兔应用价值领域。射不同浓度的细菌内毒素呈现不同的升温幅度,对细菌内毒素的敏感性与其浓度呈正相关,浓度越大,敏感性越高。通过本试验可知福建黄兔体温较为恒定,对细菌内毒素也表现出一定的敏感性,若按实验用兔标准饲养,质量将更为稳定,可以探讨应用于热原试验,以拓宽福建黄兔应用价值领域。

关键词: 碳汇, 碳汇

Abstract: To obtain the body temperature instance and sensitivity response to bacterial endotoxin of the Fujian rabbits, and to discuss whether the Fujian rabbits can be adapted to suit the use of pyrogen test. According to the standard pyrogen test from ‘Chinese Codex’, to choose Fujian rabbits with the eligible body temperature, which were administrated with three doses of endotoxin (5 Eu/mL, 10Eu/mL, 20Eu/mL) to analyze the sensitivity of these rabbits response to endotoxin. The 65.85% of the tested Fujian rabbits were up to the mustard and the body temperature of most rabbits was 38.5-39.5℃. The eligibity whereas under the Japanes White rabbit, WHBE and SPF New Zealand rabbits. The sensitivity of the Fujian rabbits reponse to bacterial endotoxin was positive correlativity to the dose of endotoxin, the big dose resulted to the high sensitivity. Through the test, it was known that most of the Fujian rabbits’ body temperature was steady, which had some sensitivity to the bacterial endotoxin. If the Fujian rabbits were fed in a standard way of laboratory animal, the quality of rabbits would more stable, and these rabbits would fit for the pyrogen test, which could broaden out the Fujian rabbits′ application field.