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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (36): 84-90.doi: 10.11924/j.issn.1000-6850.2012-3928

所属专题： 生物技术； 小麦

• 农学 农业基础科学 • 上一篇    下一篇

硅作用下镉对小麦幼苗生理生化指标的影响研究

李冬香 李光德 张华 刘明明 郝英华   

  • 收稿日期:2012-12-04 修回日期:2013-01-06 出版日期:2013-12-25 发布日期:2013-12-25

Study on the Effect of Cd on Physiological and Biochemical Characteristics of Wheat Seedling at the Action of Si

  • Received:2012-12-04 Revised:2013-01-06 Online:2013-12-25 Published:2013-12-25

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摘要： 为对镉污染区域的小麦增施硅肥提供理论依据，明确在添加不同浓度硅的作用下，镉对小麦生理生化各个指标的影响，通过采用水培试验方法，研究在不同加硅量作用下，镉对小麦幼苗叶绿素含量、抗氧化酶系统活性(SOD、POD、CAT)、脯氨酸含量、可溶性蛋?白含量以及MDA含量的影响。结果表明：在加硅量(30，60，90 mg/L)作用下，小麦幼苗的叶绿素a含量显著升高，分别为74.35%、100.00%和116.75%，叶绿素b分别升高了64.71%、48.53%和83.82%，可溶性蛋白质含量显著升高，分别为5.41%、14.68%和21.80%，SOD活性显著升高，分别为269.98%、222.46%和139.24%，MDA的含量则显著降低，分别为26.79%、34.82%和42.86%，说明硅缓解了镉毒害对小麦幼苗的影响；在加硅量(120，150 mg/L)作用下，虽然小麦幼苗叶绿素b的含量与对照相比没有显著性差异，但是叶绿素a显著提高了47.64%和38.74%、可溶性蛋白质含量显著提高了18.28%和3.48%，MDA的含量显著降低了32.14%和30.36%，依然在一定程度上缓解了镉毒害对小麦幼苗的影响；在加硅量180 mg/L作用下，虽然小麦幼苗MDA含量有所降低，但是脯氨酸和可溶性蛋白质含量与对照相比均显著降低。结论：浓度低于150 mg/L的加硅量作用下可以缓解镉对小麦幼苗的毒害。

关键词: 发芽率, 发芽率

Abstract: To provide the theory basis for increased application Si fertilizer for wheat in Cd pollution area, and to clear the influence on each wheat physiological and biochemical index of Cd under the action of Si of different concentration. The water culture experiment was conducted to investigate the effects of Cd on chlorophyll content, antioxidant enzyme system activity (SOD, POD, CAT), proline content, soluble protein content and MDA content of Triticum aestivuml with different concentrations of Si. The results showed that: at the action of Si (30, 60, 90 mg/L), chlorophyll-a content increased by 74.35%, 100.00% and 116.75%, chlorophyll-b content increased by 64.71%, 48.53% and 83.82%, soluble protein content increased by 5.41%, 14.68% and 21.80%, SOD activity of wheat seedling increased by 269.98%, 222.46% and 139.24% and MDA content droped by 26.79%, 34.82% and 42.86%, alleviating the toxicity of Cd on Triticum aestivuml; at the action of Si (120, 150 mg/L), chlorophyll-b content of Triticum aestivuml compared with the control group showed no significant difference, but chlorophyll-a content increased by 47.64% and 38.74%, soluble protein content increased by 18.28% and 3.48% and MDA content droped by 32.14% and 30.36%, alleviating the toxicity of Cd on Triticum aestivuml; at the action of Si 180 mg/L, MDA content droped, but proline content, soluble protein content of Triticum aestivuml droped. Si of the concentration less than 150 mg/L could alleviate the mischief on the wheat seedlings under the action of cadmium.

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