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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (30): 191-196.doi: 10.11924/j.issn.1000-6850.2013-0023

所属专题： 生物技术； 油料作物； 农业生态

• 植物保护 农药 • 上一篇    下一篇

黑龙江大豆栽培品种和新品系对疫霉病抗性评价及抗病基因分析

马淑梅 邵红涛 韩新华   

  • 收稿日期:2013-01-05 修回日期:2013-02-21 出版日期:2013-10-25 发布日期:2013-10-25

Resistance Evaluation and Resistance Genes analysis of soybean cultivars and new lines in Heilongjiang province to Phytophthora root rot

  • Received:2013-01-05 Revised:2013-02-21 Online:2013-10-25 Published:2013-10-25

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摘要： 由Phytophthora sojae引致的大豆疫霉病是黑龙江大豆产区的重要病害之一。该病已在我国大豆一些主要栽培区发生，并引起较大危害。培育和种植抗疫霉病品种是控制该病最有效的方法。本研究旨在筛选黑龙江地区的大豆疫霉病抗病品种和品系，为病害的防治和抗病品种的合理布局提供参考。在大豆苗期用下胚轴伤口接种方法对126个栽培大豆品种和135份大豆品系进行接种，鉴定其对黑龙江大豆主要疫霉病菌株（8个）的抗性。鉴定结果表明：有72个品种抗4个以上菌株，占鉴定品种的57.1%；84份品系抗4个以上菌株，占鉴定品系的62.2%。对品种的基因分析表明，有30个大豆品种含有抗病基因，其中有10个品种分别含有Rps1k、 Rps3a、Rps1c三个主要基因。

关键词: 体育行为, 体育行为

Abstract: Phytophthora root rot caused by Phytophthora sojae is one of main diseases in Heilongjiang soybean production. This disease have happened in the main soybean production areas of our country and led to a large quantity of outputs lose in some areas. Planting resistant cultivar is the most effective methods. So this research was focused on screening Phytophthora root rot resistant cultivars and resistance lines in Heilongjiang areas, and offered reference for controlling local disease and rational layout of disease-resistant cultivars. We used the hypocotyl vulnus inoculum to vaccinate 126 soybean cultivars and 135 portions of soybean lines, in order to identify eight main Phytophthora sojae strains. Our results showed that 72 portions of cultivars are resistant to more than 4 strains, occupy 57.1% of identified cultivars, and 84 portions of lines are resistant to more than 4 strains, occupy 62.2% of identified lines. Genes analysis of cultivars indicated that 30 soybean cultivars contained disease-resistant genes, 3 major genes, such as Rps1k, Rps3a and Rps1c, are included in 10 cultivars.