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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (27): 58-63.doi: 10.11924/j.issn.1000-6850.2013-0591

所属专题： 小麦

• 农学 农业基础科学 • 上一篇    下一篇

小麦秸秆不同部位水浸液对牛筋草的化感作用研究

刘小民 边全乐 李秉华 樊翠芹 许贤 王贵启 梁双波   

  • 收稿日期:2013-03-05 修回日期:2013-03-28 出版日期:2013-09-25 发布日期:2013-09-25
  • 基金资助:
    国家科技支撑计划

Allelopathic effects of aqueous extracts of different segments of wheat straw on the seed germination and seedling growth of goosegrass(Eleusine indica).

  • Received:2013-03-05 Revised:2013-03-28 Online:2013-09-25 Published:2013-09-25

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摘要： 通过室内生物测定法研究了小麦秸秆的不同部位（根、茎、穗）不同浓度水浸液（6.25，12.5，25，50，100mg?mL-1）对牛筋草种子萌发和幼苗生长的化感作用，结果表明：小麦秸秆根、茎、穗部水浸液对牛筋草种子的萌发均具有抑制作用，随着浓度的升高，牛筋草发芽率逐渐降低，发芽指数逐渐减少，以最高浓度100mg?mL-1水浸液抑制作用最强，不同部位水浸液处理的牛筋草发芽率依次为对照（84.5%）＞根（71.3%）＞茎（68.7%）＞穗（58.7%），发芽指数依次为对照（18.39）＞根（12.82）＞茎（6.77）＞穗（6.07）。根部水浸液对牛筋草苗高无显著影响，茎、穗部水浸液只有在最高浓度处理下显著抑制其幼苗高度，但是对根部生长均表现出“低促高抑”作用，当根、茎、穗部水浸液浓度为6.25mg?mL-1时，牛筋草根长分别比对照增加17.1%，17.8%及6.6%，而当其浓度为100mg?mL-1时，牛筋草根长分别比对照降低25.6%、31.6%及95.6%。通过拟合剂量-效应曲线得到小麦秸秆根、茎及穗部水浸液对牛筋草根部生长的半数效应浓度EC50值分别为219.182，157.545及41.007mg?mL-1

关键词: 酵母双杂交, 酵母双杂交

Abstract: Allelopathic effects of aqueous extracts from root, stem and spikes of wheat straw on the seed germination and seedling growth of goosegrass (Eleusine indica) were tested with a petri dish bioassay method. The results indicated that they all inhibited the germination of goosegrass, germination rate and index decreased with the increasing concentration, and the treatment of 100mg?mL-1 had the strongest inhibition effect. Germination rate of root, stem and spikes aqueous extracts was 71.3%, 68.7% and 58.7% respectively, while the control was 84.5%, and germination index was 12.82, 6.77, 6.07 respectively, while the control was 18.39. Stem and spikes aqueous extracts inhibited the shoot length of goosegrass significantly only at the highest concentration, while root aqueous extracts had little effects. The root, stem and spikes aqueous extracts promoted the root growth of goosegrass at low concentrations but inhibited it at high concentrations. Compared with control, the root length increased by 17.1%，17.8% and 6.6% respectively when the concentration of root, stem and spikes aqueous extracts was 6.25mg?mL-1, but decreased by 25.6%、31.6% and 95.6% respectively when the concentration was 100mg?mL-1. EC50 was calculated through dose-response equation between different concentrations of root, stem and spikes aqueous extracts and root lengths, and the value was 219.182，157.545 and 41.007mg?mL-1 respectively